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3D-Printed SARS-CoV-2 RNA Genosensing Microfluidic System
(Wiley, 2022-02-05) González Crevillén, Agustín; Mayorga-Martinez, Carmen C.; Vaghasiya, Jayraj V.; Martin Pumera
Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab-on-a-chip device through electrochemical detection using 3D printing technology for SARS-CoV-2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D-printed by a 3D printing pen (3D-PP). The 3D-PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS-CoV-2. The sensing mechanism relies on the electro-oxidation of adenines present in ssDNA when in contact with SARS-CoV-2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS-CoV-2/3D-PP genosensor shows high sensitivity and fast response.
3D-printed Transmembrane Glycoprotein Cancer Biomarker Aptasensor
(Elsevier, 2021-08-21) González Crevillén, Agustín; Mayorga Martinez, Carmen C.; Zelenka, Jaroslav; Rimpelová, Silvie; Ruml, Tomáš; Pumera, Martin
Point-of-care, easy to manufacture, and low cost detection of cancer biomarkers is crucial for fighting the early stage disease. 3D printing allows delocalized printing at remote locations with updates/upgrades available globally via electronic files. Mucin 1 (MUC1) is a protein that is overexpressed in a number of epithelial cancers and is a key factor in advancement of the disease. Here we show the simple point-of-care 3D printing fabrication of a MUC1 aptasensor using a nanocarbon/polymer filament. Efficacy of the 3D-printed aptasensor is demonstrated by monitoring the expression and release of MUC1 in breast cancer cell cultures. Such a simple, low cost, and easy to locally fabricate cancer biosensor will have a large impact on the field of cancer diagnostics.
A pan-cheloniid turtle from the Middle Miocene of Portugal
(Wiley, 2024) Pérez García, Adán; Telles Antunes, Miguel
Currently, there is no information on the fossil record of Pan-Cheloniidae from the Neogene of the Iberian Peninsula. A well-preserved partial skeleton attributable to this lineage of turtles, from the Middle Miocene of Portugal, is presented here. It preserves much of the anterior half of its carapace, in which the plates remain articulated, as well as several articulated dorsal vertebrae, and an isolated cervical and a caudal vertebrae. The analysis of this Serravallian find shows that it cannot be attributed to a hitherto described taxon. Thus, a new member of Pan-Cheloniidae is defined, Lusochelys emilianoi gen. et sp. nov., improving the relatively limited knowledge about this lineage for the Middle Miocene global record. It represents the first generic and specific systematic attribution for a member of Pan-Chelonioidea in the Neogene record of the Iberian Peninsula.
Abelian Actions on Pseudo-real Riemann Surfaces
(Springer, 2023-04-08) Bujalance García, Emilio; Cirre Torres, Francisco Javier; J. Rodríguez
A compact Riemann surface is called pseudo-real if it admits orientation-reversing automorphisms but none of them has order two. In this paper, we find necessary and sufficient conditions for the existence of an action on a pseudo-real surface of genus g 2 of an abelian group containing orientation-reversing automorphisms. Several consequences are obtained, such as the solution of the minimum genus problem for such abelian actions.
Absorbing Markov decision processes
(EDP Sciences, 2024-02-09) Dufour, François; Prieto Rumeau, Tomás
In this paper, we study discrete-time absorbing Markov Decision Processes (MDP) with measurable state space and Borel action space with a given initial distribution. For such models, solutions to the characteristic equation that are not occupation measures may exist. Several necessary and sufficient conditions are provided to guarantee that any solution to the characteristic equation is an occupation measure. Under the so-called continuity-compactness conditions, we first show that a measure is precisely an occupation measure if and only if it satisfies the characteristic equation and an additional absolute continuity condition. Secondly, it is shown that the set of occupation measures is compact in the weak-strong topology if and only if the model is uniformly absorbing. Several examples are provided to illustrate our results.
Accurate Test of Chiral Dynamics in the → 𝛾 ⁢𝑝 →𝜋0⁢𝑝 Reaction
(American Physical Society, 2013-08-07) Hornidge, D; Aguar Bartolomé, P.; J. R. M. Annand,; Fernández Ramírez, César; et al
A precision measurement of the differential cross sections 𝑑⁢𝜎/𝑑⁢𝛺 and the linearly polarized photon asymmetry 𝛴 ≡(𝑑⁢𝜎⊥ −𝑑⁢𝜎∥)/(𝑑⁢𝜎⊥ +𝑑⁢𝜎∥) for the →𝛾⁢𝑝 →𝜋0⁢𝑝 reaction in the near-threshold region has been performed with a tagged photon beam and almost 4⁢𝜋 detector at the Mainz Microtron. The Glasgow-Mainz photon tagging facility along with the Crystal Ball/TAPS multiphoton detector system and a cryogenic liquid hydrogen target were used. These data allowed for a precise determination of the energy dependence of the real parts of the 𝑆- and all three 𝑃-wave amplitudes for the first time and provide the most stringent test to date of the predictions of chiral perturbation theory and its energy region of agreement with experiment.
Addition reaction of azoles to acetone-d 6: NMR and computational studies
(Wiley, 2024-05-22) Claramunt Vallespí, Rosa María; Sanz del Castillo, Dionisia; Alkorta, Ibon; Elguero, José
The reactivity of imidazole, pyrazole, 1,2,4-triazole, 1,2,3-triazole and tetrazole with acetone (propan-2-one) has been studied by 1 H and 13 C NMR using acetone-d 6 as solvent at temperatures ranging from 173 to 300 K at 10 K intervals. Simultaneously, the reaction has been theoretically calculated at the B3LYP/6-311++G(d,p) level and experimental and theoretical results compared. The equilibrium constants between azoles and adducts α,α-dimethyl-azole-methanol were analyzed assuming that the straight part of the plots –R ln Ke vs. 1/T can be used to determine ΔH and ΔS. Calculated and experimental data are related but the theoretical values are proportionally higher. The tautomerism of triazoles and tetrazole has been considered in order to discuss the reactions.
An extreme wave event in Timanfaya National Park: Possible first geological evidence of the 1755 Lisbon tsunami in Lanzarote, Canary Islands
(Wiley, 2024-10) Galindo Jimenez, Inés; Romero, Carmen; Martín-González, Esther; Sanchez, Nieves; Vegas, Juana; Lario Gómez, Javier; https://orcid.org/0000-0002-2545-5234; https://orcid.org/0000-0002-9125-2275; https://orcid.org/0000-0001-5659-2197; https://orcid.org/0000-0001-9239-5254; https://orcid.org/0000-0001-9137-0020
The identification of extreme wave events' deposits is of the main importance in the contexts of global warming and coastal geohazards. Specifically, improving the knowledge of this phenomenon is extremely relevant for high populated volcanic oceanic islands. In this paper, we analyse two extreme wave event deposits located on a coastal platform formed by lavas from the 1730–1736 Timanfaya eruption in Lanzarote Island (Spain). The first one consists of a boulder ridge parallel to the coast of approximately 750 m in length and 7 m asl in elevation. These are accumulations of non-cemented large boulders and sands that extend about 150 m inland from the intertidal zone. The boulders are of basaltic composition, heterometric, sub-rounded to angular, and they reach sizes up to 3 m of major axis. They are imbricated both inland and seaward, indicating a strong inundation and backwash. The second deposit is a small outcrop of boulders of equal composition and sizes up to 1 m of major axis, reaching an elevation up to 6 m asl, and has been correlated with the former deposit. Here, the boulders were also deposited on the Timanfaya lavas and later covered by lava flows extruded during the 1824 eruption. Therefore, both deposits could be related with a chronologically well-contrasted event, between 1736 and 1824. The origin of these deposits could be interpreted as an extreme storm or a tsunami. There are no historical records of extreme storms in the Canary Islands for this period, but there is documentary evidence of the tsunamis of 1761 and 1755. Moreover, for the latter, there is documentation that indicates its impact on coastal infrastructures in the Canary Islands, including the western slope of Lanzarote, and therefore, we propose these deposits as the first sedimentary evidence of the 1755 tsunami in the Canary Islands.
Analysis of smart thermostat thermal models for residential building
(Elsevier, 2022-06-02) Arias, J.; Khan, A.A.; Rodriguez Uría, J.; Sama Meige, Miguel Ángel
This work studies the thermal behavior of residential buildings by using the data provided by smart thermostats and weather forecast data. We consider an equivalent circuit model depending on four parameters related to the heater power, the solar energy, heat capacity, and the thermal resistance of the building. We employ a random ordinary differential equation to overcome the natural model uncertainty. We develop a differential equation constrained least-squares-based parameter identification approach for uncertainty quantification. We provide results related to the analytic properties of the parameter-to-solution map involving a derivative characterization. Furthermore, using a discretization scheme adapted to the given data, we derive discrete formulas for the deterministic identification model and compute the statistical moments of the random model. Following a machine learning approach, we propose an algorithm that consists of three phases. The first is a training phase where we identify the parameter uncertainties on a training dataset. In the second phase, we establish a normal distribution of the parameters using these uncertainties. In the final phase, we simulate the temperature on a test dataset by solving the random model. We test this algorithm on real data from two residential buildings. The detailed numerical experiments show the feasibility and the efficacy of the developed framework.
Analysis of total arsenic content in purchased rice from Ecuador
(Czech Academy of Agricultural Sciences, 2019-12-31) Atiaga Franco, Oliva L.; Otero, Xose L.; Gallego Picó, Alejandrina; Escobar Castañeda, Luis A.; Carrera Villacrés, David; Bravo Yagüe, Juan Carlos
Natural and anthropogenic sources contribute to arsenic contamination in water and human food chain in Andean countries. Human exposure to arsenic via rice consumption is of great concern in countries where this crop is the dominant staple food, and limited information is available on the arsenic contamination on rice in Ecuador. This work was to contribute to the lack of knowledge analysing total arsenic by hydride generation-atomic absorption spectrometry in the samples of white, brown and parboiled rice purchased in Ecuadorian markets and produced in the two main rice wetlands in Ecuador, Guayas and Los Ríos, were carried out. For the samples from Guayas, arsenic concentration in white, brown and parboiled rice were 0.174 ± 0.014, 0.232 ± 0.021, and 0.186 ± 0.017 mg/kg respectively, whereas samples of white rice from Los Ríos showed a total arsenic level of 0.258 ± 0.037 mg/kg. This last arsenic concentration exceeds recommended maximum permissible limit by the FAO/WHO. Obtained data have available to estimate the Ecuadorian dietary exposure revealing serious health risk for population
Asymptotically Linear Problems and Antimaximum Principle for the Square Root of the Laplacian
(2016-03-10) Arcoya, David; Colorado, Eduardo; Leonori, Tommaso; https://orcid.org/0000-0002-7284-2413; https://orcid.org/0000-0002-1067-5752; https://orcid.org/0000-0002-0848-4463
This work deals with bifurcation of positive solutions for some asymptotically linear problems, involving the square root of the Laplacian (-Delta)(1/2). A simplified model problem is the following: {(-Delta)(1/2)u = lambda m(x)u + g(u) in Omega, u = 0 on partial derivative Omega, with Omega subset of R-N a smooth bounded domain, N >= 2, lambda > 0, m is an element of L-infinity(Omega), m(+) not equivalent to 0 and g is a continuous function which is super-linear at 0 and sub-linear at infinity. As a consequence of our bifurcation theory approach we prove some existence and multiplicity results. Finally, we also show an anti-maximum principle in the corresponding functional setting.
Basic estimates for solutions of a class of nonlocal elliptic and parabolic equations
(American Institute of Mathematical Sciences (AIMS), 2015-12) Leonori, Tommaso; Peral, Ireneo; Primo, Ana; Soria, Fernando; https://orcid.org/0000-0002-0848-4463; https://orcid.org/0000-0003-2297-9910; https://orcid.org/0000-0003-1804-3175; https://orcid.org/0000-0001-5753-807X
In this work we consider the problems { script Lu = f in Ω, u = 0 in ℝN\Ω, and { ut + script Lu = f in QT ≡ Ω x (0,T), u(x,t) = 0 in (ℝN\Ω) x (0,T), u(x, 0) = 0 in Ω, where script L is a nonlocal differential operator and Ω is a bounded domain in ℝN, with Lipschitz boundary. The main goal of this work is to study existence, uniqueness and summability of the solution u with respect to the summability of the datum f. In the process we establish an Lp-theory, for p ≥ 1, associated to these problems and we prove some useful inequalities for the applications.
Basolites: A type of Metal Organic Frameworks highly efficient in the one-pot synthesis of quinoxalines from α-hydroxy ketones under aerobic conditions
(Elsevier, 2020-04-01) Godino Ojer, Marina; Shamzhy, Mariya; Čejka, Jiři; Pérez Mayoral, María Elena
We report here efficient synthesis of quinoxalines from o-phenylendiamine and α-hydroxy ketones over commercial Basolites. The concentration and type of acid centres, as CUS sites, together with the porosity of the samples strongly influence the resulting conversion and selectivity. Our results indicate that the formation of quinoxalines preferentially follows a new route, in which tautomerization reactions are also involved.
Behavioural and biochemical alterations by chlorpyrifos in aquatic insects: an emerging environmental concern for pristine Alpine habitats
(Springer Nature, 2019-10-19) Di Nica, Valeria; Muñiz González, Ana Belén; Lencioni, Valeria; Villa, Sara; Springer Nature; https://orcid.org/0000-0002-4341-9923
This study aimed to assess how different concentrations of the insecticide chlorpyrifos (1.1, 5.24, 11, 52.4, 110, 262, 524 and 1100 ng L−1 ) affect the swimming behaviour of Diamesa zernyi larvae following exposure. A video tracking system was employed to analyse two swimming traits (total distance moved and average speed) of the larvae simultaneously after 3 days of exposure to the pesticide at 2 °C. The behavioural results were also interpreted according to biochemical responses to oxidative stress (OS) induced by chlorpyrifos, based on malondialdehyde (MDA) and protein carbonyl (PCC) content. Both distance and speed significantly decreased after 72 h of exposure to chlorpyrifos concentrations of ≥ 110 ng L−1 , under which significant OS was detected as lipid peroxidation (level of MDA) and protein carbonylation (level of carbonyl). Analysis of altered swimming behaviour, along with MDA and carbonyl content, indicated that ≥ 110 ng L−1 contamination levels of the insecticide cause the organism to reallocate energy normally used for locomotor activity to repair cell damage, which might explain the strong impairment to locomotor performance. Locomotor performance is an ecologically relevant trait for elucidating the population dynamics of key species, with disturbance to this trait having long-term negative impacts on population and community structure. Therefore, chlorpyrifos insecticides represent a serious ecological risk for mountain aquatic species based on the detrimental effects observed in the current study, as the tested concentrations were those at which the insecticide is found in many Alpine rivers of Italy.
A bothremydid turtle (Pleurodira) from the middle Cenomanian of Vale de Figueira (Belas, Portugal)
(Wiley, 2024) Telles Antunes, Miguel; Pérez García, Adán
An unpublished turtle shell from the middle Cenomanian of Vale de Figueira, near Belas (Lisbon District, Portugal), is recognized by us as collected in 1880 under the direction of Carlos Ribeiro. No turtle remains from that region had so far been figured, described or discussed from a systematic point of view. The specimen corresponds to a partial but articulated shell. It is attributed to Pleurodira and, more specifically, to Bothremydidae. Only one pre-Campanian turtle specimen was previously identified at the infrafamilial level in the Cretaceous record of Portugal. It was a partial shell from the middle Cenomanian of Nazaré (Leiria District) attributed to the bothremydid Algorachelus peregrina, a species defined in a Spanish synchronous locality (Algora, in Central Spain). Several anatomical regions in the specimen from Vale de Figueira were not preserved in the partial carapace from Nazaré, and differences in the morphology of some elements are recognized between both shells. However, the individual studied here is also ascribed to Algorachelus peregrina, these differences being justified by intraspecific variability. Therefore, the specimen represents the second evidence on the species in Portugal, being the only one recognized for the Lisbon District.
Changes in littoral dynamics and sea level in southern Iberia and Canary Istands during the Holocene.
(Sociedad Geológica de España, 1996) Zazo Cardeña, Caridad; Dabrio, Cristino José; Goy Goy, José Luis; Bardají Azcárate, Teresa; Ghaleb, B.; Lario Gómez, Javier
The interaction between global factors tglobal" glacio-eustatic sea-leve/ rise) and regional factors (tectonic trend, oceanographic factor) is analyzed in different areas of the lberian and Canary lsland littora/. Sea-leve/ changes and climatic variations are stated for the Mediterranean and Atlantic littora/s, with different tidal ranges and tectonic trends. Different stages can be envisaged in the coastal evolution of a/most ali the analyzed areas from the beginning of Holocene (10.000 BP): at ca. 7.000 BP (Flandrian maximum) with a change from open estuaries to aggradational processes (Cádiz Gulf, subsiding area), basal peat (Valencia Gulf, subsiding area) and development of microcliffs (Mediterranean uplifting areas). From 7,000 to 2,700 BP a first progradation phase with development of spit bars took place in the mediterranean uplifting areas, whilst in the atlantic subsiding one starts the aggradation processes in the formerly open estuaries, an a/so from ca.4,000 BP development of spit-bar system. A second progradation phase ofthe spit bars systems occurs between 2,400 and 500 BP both in Almería and Cádiz Gulf, where a change from aggradation to progradation takes place in the estuary environments, and with a change in the direction of /ongshore drift (Mediterranean) and prevailing winds (both areas) at ca.2,400 BP. At ca. 1,200 BP a sudden paleogeographic change took place in estuary environments. From 500 BP up to now a marked increase of coastal progradation in ali littorals is observed. Climatic interpretations /ed to corre/ate anticyclonic conditions (as it happened during Little Ice Ages) with the growing of spit bar systems and progradation, while low pressure conditions (as during Medieval Warm Period) coincides with no progradation. In the Canary lslands, the development of storm ridges at ca. 3,500 BP can be corre/ated with an intensification of trade winds.
Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress
(ELSEVIER, 2023) Llorente Ortega, Lola; Aquilino Amez, Mónica; Herrero Felipe, Óscar; Peña, Eduardo de la; Planello Carro, María del Rosario; https://orcid.org/0000-0002-1716-6889; https://orcid.org/0000-0002-6057-6664
This paper characterizes the heat stress response (HSR) and explores the impact of temperatures on the immune response of larvae from two chironomid species, Prodiamesa olivacea and Chironomus riparius. Genes involved in crucial metabolic pathways were de novo identified in P. olivacea: Hsp27, Hsp60, Hsp70, Hsc70, Cdc37, and HSF for the heat stress response (HSR) and TOLL, PGRP, C-type lectin, and JAK/hopscotch for the immune system response (ISR). Quantitative real-time PCR was used to evaluate the expression levels of the selected genes in short-term treatments (up to 120') at high temperatures (35 °C and 39 °C). Exposing P. olivacea to elevated temperatures resulted in HSR induction with increased expression of specific heat shock genes, suggesting the potential of HSPs as early indicators of acute thermal stress. Surprisingly, we found that heat shock represses multiple immune genes, revealing the antagonist relation between the heat shock response and the innate immune response in P. olivacea. Our results also showed species-dependent gene responses, with more significant effects in P. olivacea, for most of the biomarkers studied, demonstrating a higher sensitivity in this species to environmental stress conditions than that of C. riparius. This work shows a multi-species approach that enables a deeper understanding of the effects of heat stress at the molecular level in aquatic dipterans.
Characterization of the detrimental effects of type IV glandular trichomes on the aphid Macrosiphum euphorbiae in tomato
(Wiley, Society of Chemical Industry, 2021) Lidia Blanco Sánchez; Planello Carro, María del Rosario; Llorente Ortega, Lola; Díaz Pendón, Juan Antonio; Ferrero, Victoria; Fernandez Muñoz, Rafael; Herrero Felipe, Óscar; Peña, Eduardo de la; https://orcid.org/0000-0002-1716-6889; https://orcid.org/0000-0002-3616-6622; https://orcid.org/0000-0002-2091-8957; https://orcid.org/0000-0002-4054-8949; https://orcid.org/0000-0002-6057-6664
BACKGROUND Glandular trichomes are essential in plants' defence against pests however, the mechanisms of action are not completely understood. While there is considerable evidence of feeding and movement impairment by trichomes, the effect on other traits is less clear. We combined laboratory and greenhouse experiments with molecular analysis to understand how glandular trichomes affect the behavior, population growth, and the expression of biomarkers involved in detoxification, primary metabolism, and developmental pathways of the aphid Macrosiphum euphorbiae. We used two isogenic tomato lines that differ in the presence of type IV glandular trichomes and production of acylsucroses; i.e.,Solanum lycopersicum cv. ‘Moneymaker’ and an introgressed line from Solanum pimpinellifolium (with trichomes type IV). RESULTS Type IV glandular trichomes affected host selection and aphid proliferation with aphids avoiding, and showing impaired multiplication on the genotype with trichomes. The exposure to type IV glandular trichomes resulted in the overexpression of detoxication markers (i.e., Hsp70, Hsp17, Hsp10); the repression of the energetic metabolism (GAPDH), and the activation of the ecdysone pathway; all these, underlying the key adaptations and metabolic trade-offs in aphids exposed to glandular trichomes. CONCLUSION Our results demonstrate the detrimental effect of glandular trichomes (type IV) on the aphid and put forward their mode of action. Given the prevalence of glandular trichomes in wild and cultivated Solanaceae; and of the investigated molecular biomarkers in insects in general, our results provide relevant mechanisms to understand the effect of trichomes not only on herbivorous insects but also on other trophic levels.
Charmonium spectrum with a Dirac potential model in the momentum space
(American Physical Society, 2017-05-26) Molina, David; Sanchis, Maurizio De; Fernández Ramírez, César
We study the charmonium spectrum using a complete one gluon exchange approach based on a phenomenological relativistic 𝑞⁢¯𝑞 potential model with Dirac spinors in momentum space. We use phenomenological screening factors to include vacuum quantum effects. Our formulation does not rely on nonrelativistic approximations. We fit the lowest-lying charmonia (below the 𝐷⁢¯𝐷 threshold) and predict the higher-lying resonances of the spectrum. In general, we reproduce the overall structure of the charmonium spectrum and, in particular, we can reasonably describe the 𝑋⁡(3872) resonance mass as (mostly) a 𝑐⁢ ¯𝑐state. The numerical values of the free parameters of the model are determined taking into account also the experimental uncertainties of the resonance energies. In this way, we are able to obtain the uncertainties of the theoretical resonance masses and the correlation among the free parameters of the model.
Chironomus riparius exposure to field-collected contaminated sediments: From subcellular effect to whole-organism response
(ELSEVIER, 2019) Arambourou, Hélène; Planello Carro, María del Rosario; Llorente, Lola; Fuertes, Inmaculada; Barata, Carlos; Delorme, Nicolas; Noury, Patrice; Herrero Felipe, Óscar; Villeneuve, Aurélie; Bonnineau, Chloe; https://orcid.org/0000-0003-0590-9072; https://orcid.org/0000-0002-1716-6889; https://orcid.org/0009-0001-9185-5105; https://orcid.org/0000-0001-6341-003X
The toxicity of three field-collected sediments differentially contaminated with pesticides, heavy metals, phtalates and polycyclic aromatic hydrocarbons (PAHs), was assessed in Chironomus riparius. For this purpose, C. riparius larvae were exposed throughout their entire life cycle to sediments collected in three sites along the Saulx river in France, and the toxic effects were measured at different levels of biological organization: from the molecular (lipidomic analysis and transcriptional variations) to the whole organism response (respiration rate, shape markers and emergence rate). In the sediment characterized by an intermediate level of contamination with PAHs and phtalates, we detected an increase of the cell stress response and delayed emergence of males. In the group exposed to the most contaminated sediment with PAHs, phtalates and pesticides, genes related to endocrine pathways, cell stress response and biotransformation processes were overexpressed, while female wing shape was affected. Field-collected sediment exposure did not induce significant effects on mentum shape markers or on the lipid profile. The present study provides new insights into the multilevel effects of differentially contaminated sediments in insects. This integrative approach will certainly contribute to improved assessment of the risk that complex mixtures of pollutants pose to the aquatic ecosystem.

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