Publicación: Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress
Fecha
2023
Autores
Llorente Ortega, Lola
Aquilino Amez, Mónica
Peña, Eduardo de la
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info:eu-repo/semantics/openAccess
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Resumen
This paper characterizes the heat stress response (HSR) and explores the impact of temperatures on the immune response of larvae from two chironomid species, Prodiamesa olivacea and Chironomus riparius. Genes involved in crucial metabolic pathways were de novo identified in P. olivacea: Hsp27, Hsp60, Hsp70, Hsc70, Cdc37, and HSF for the heat stress response (HSR) and TOLL, PGRP, C-type lectin, and JAK/hopscotch for the immune system response (ISR). Quantitative real-time PCR was used to evaluate the expression levels of the selected genes in short-term treatments (up to 120') at high temperatures (35 °C and 39 °C). Exposing P. olivacea to elevated temperatures resulted in HSR induction with increased expression of specific heat shock genes, suggesting the potential of HSPs as early indicators of acute thermal stress. Surprisingly, we found that heat shock represses multiple immune genes, revealing the antagonist relation between the heat shock response and the innate immune response in P. olivacea. Our results also showed species-dependent gene responses, with more significant effects in P. olivacea, for most of the biomarkers studied, demonstrating a higher sensitivity in this species to environmental stress conditions than that of C. riparius. This work shows a multi-species approach that enables a deeper understanding of the effects of heat stress at the molecular level in aquatic dipterans.
Descripción
The registered version of this article, first published in “Ecotoxicology and Environmental Safety, 2023", is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.ecoenv.2023.115359
La versión registrada de este artículo, publicado por primera vez en “Ecotoxicology and Environmental Safety, 2023", está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.ecoenv.2023.115359
Categorías UNESCO
Palabras clave
chironomids, transcriptional biomarkers, immune system response, heat shock response, global change, sentinel organism
Citación
Lola Llorente, Mónica Aquilino, Óscar Herrero, Eduardo de la Peña, Rosario Planelló, Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress, Ecotoxicology and Environmental Safety, Volume 263, 2023, 115359, ISSN 0147-6513, https://doi.org/10.1016/j.ecoenv.2023.115359
Centro
Facultades y escuelas::Facultad de Ciencias
Departamento
Física Matemática y de Fluídos