Publicación: Activity-based anorexia alters hypothalamic POMC and orexin populations in male rats
Fecha
2022-08-11
Autores
Pinos, Helena
Sánchez Serrano, Ricardo
Fernández García, José Manuel
Paz Regidor, Ana María de
López Tolsa Gómez, Gabriela Eugenia
Vidal García, Pedro
Gutiérrez Ferre, Valeria Edith
Pellón Suárez de Puga, Ricardo
Editor/a
Director/a
Tutor/a
Coordinador/a
Prologuista
Revisor/a
Ilustrador/a
Derechos de acceso
info:eu-repo/semantics/openAccess
Título de la revista
ISSN de la revista
Título del volumen
Editor
Elsevier
Resumen
The objective of this study was to investigate the orexin and POMC populations in the hypothalamic nuclei of male Wistar rats after the activity-based anorexia (ABA) procedure. Four groups were established based on food restriction and activity: activity (A), ABA, diet (D) and control (C). The ABA protocol consisted of free access to a running wheel for a period of 22 h and access to food for 1 h. When the animals in the ABA group reached the ABA criterion, were sacrificed, and their brains were collected and serially sectioned. The free-floating sections were processed for orexin and POMC immunostaining. The number of orexin A-ir cells in the perifornical-dorsomedial-hypothalamus continuum (PFD) and lateral hypothalamus (LH) and the number of POMC-ir cells in the arcuate nucleus (Arc) were estimated. Data on food intake, body weight and wheel turns were also analyzed. The ABA procedure caused a significant decrease in body weight along with a significant increase in activity. Moreover, at the end of the ABA procedure, the number of POMC-ir cells decreased in the Arc in the A group, and significantly more in the ABA group, and the number of orexin A-ir positive cells decreased in the LH in D and ABA groups. The differential decrease in POMC in the ABA group emphasizes the importance of the melanocortin system in the maintenance of ABA, but more research is needed to elucidate the involvement of this peptide in the mechanism that promotes and maintains anorexia nervosa and how increased activity may interact with all these processes.
Descripción
La versión registrada de este artículo, publicado por primera vez en Behavioural Brain Research, Volume 436, 2023, 114055, ISSN 0166-4328, está disponible en línea en el sitio web del editor: https://doi.org/10.1016/j.bbr.2022.114055.
The registered version of this article, first published in Behavioral Brain Research, Volume 436, 2023, 114055, ISSN 0166-4328, is available online at the publisher's website: https://doi .org/10.1016/j.bbr.2022.114055.
Categorías UNESCO
Palabras clave
Activity-based anorexia, POMC, orexin, arcuate nucleus, male rat, lateral hypothalamus
Citación
Pinos H., Sánchez-Serrano R., Carrillo B., Fernández-García JM., García-Úbeda R., De Paz A., López-Tolsa GE., Vidal P., Gutiérrez-Ferre V., Pellón R., Collado P. (2023) Activity-based anorexia alters hypothalamic POMC and orexin populations in male rats. Behav. Brain Res., 436: 114055. FI: 3.352; Q2, https://doi.org/10.1016/j.bbr.2022.114055
Centro
Facultad de Psicología
Departamento
Psicobiología