Publicación:
Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients

Vista sencilla de ítem
dc.contributor.authorDortez, Silvia
dc.contributor.authorDeGregorio-Rocasolano, Núria
dc.contributor.authorMillán, Mònica
dc.contributor.authorGasull, Teresa
dc.contributor.authorGonzález Crevillén, Agustín
dc.contributor.authorEscarpa, Alberto
dc.date.accessioned2025-10-21T11:05:42Z
dc.date.available2025-10-21T11:05:42Z
dc.date.issued2023-07-23
dc.descriptionThe registered version of this article, first published in “Analytical Chemistry 2023, 95, 33, 12391–12397", is available online at the publisher's website: ACS, https://doi.org/10.1021/acs.analchem.3c01982 La versión registrada de este artículo, publicado por primera vez en “Analytical Chemistry 2023, 95, 33, 12391–12390", está disponible en línea en el sitio web del editor: ACS, https://doi.org/10.1021/acs.analchem.3c01982
dc.description.abstractFor the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously measured on the same PAD using ferrozine as a chromophore and a smartphone as the color reader. To this end, Tf was first isolated from serum using anti-Tf immunomagnetic beads to ensure that only the Tf-bound iron was measured, improving the selectivity and accuracy of TSAT assessment. To demonstrate the practical utility of the device, it was validated by analyzing a certified reference material, showing excellent accuracy (Er < 4%) and good precision (RSD ≤ 6%). Finally, 18 diagnosed serum samples from ischemic stroke patients were analyzed by this approach, and the results were compared with those obtained by urea-PAGE, showing not only an excellent correlation (r = 0.93, p < 0.05) but that the PAD approach has become statistically identical to the free-interference urea-PAGE. In comparison with the slow, tedious, and non-miniaturized-PAGE, this PAD approach exhibited attractive characteristics such as low cost, disposability, and connectivity, showing great potential for future point-of-care testing, especially in developing countries and/or remote areas, where access to medical or clinical facilities is limited.en
dc.description.versionversión publicada
dc.identifier.citationDortez, S., DeGregorio-Rocasolano, N., Millán, M., GasullAgustín, T., G. Crevillen, A., Escarpa, A.(2023).Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients. Analytical Chemistry 2023, 95, 33, 12391–12397. https://doi.org/10.1021/acs.analchem.3c01982
dc.identifier.doihttps://doi.org/10.1021/acs.analchem.3c01982
dc.identifier.issneISSN: 1520-6882
dc.identifier.urihttps://hdl.handle.net/20.500.14468/30549
dc.journal.issue33
dc.journal.titleAnalytical Chemistry
dc.journal.volume95
dc.language.isoen
dc.page.final12397
dc.page.initial12391
dc.publisherACS
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/deed.es
dc.subject2301 Química analítica
dc.subject2306 Química orgánica
dc.subject.keywordsColoren
dc.subject.keywordsColorimetryen
dc.subject.keywordsIron Serumen
dc.subject.keywordsTesting and assessmenten
dc.titlePaper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patientsen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication0f39284a-d95a-443a-bb2e-21297ec40b76
relation.isAuthorOfPublication.latestForDiscovery0f39284a-d95a-443a-bb2e-21297ec40b76
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Gonzalez_Crevillen_agustin_Paper_based analyt_AGUSTIN GONZALEZ CRE.pdf
Tamaño:
2.79 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
3.62 KB
Formato:
Item-specific license agreed to upon submission
Descripción:
Descargar
Colecciones
Artículos y papers