Fecha
2023-07-23
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info:eu-repo/semantics/openAccess
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ACS
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Resumen
For the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously measured on the same PAD using ferrozine as a chromophore and a smartphone as the color reader. To this end, Tf was first isolated from serum using anti-Tf immunomagnetic beads to ensure that only the Tf-bound iron was measured, improving the selectivity and accuracy of TSAT assessment. To demonstrate the practical utility of the device, it was validated by analyzing a certified reference material, showing excellent accuracy (Er < 4%) and good precision (RSD ≤ 6%). Finally, 18 diagnosed serum samples from ischemic stroke patients were analyzed by this approach, and the results were compared with those obtained by urea-PAGE, showing not only an excellent correlation (r = 0.93, p < 0.05) but that the PAD approach has become statistically identical to the free-interference urea-PAGE. In comparison with the slow, tedious, and non-miniaturized-PAGE, this PAD approach exhibited attractive characteristics such as low cost, disposability, and connectivity, showing great potential for future point-of-care testing, especially in developing countries and/or remote areas, where access to medical or clinical facilities is limited.
Descripción
The registered version of this article, first published in “Analytical Chemistry 2023, 95, 33, 12391–12397", is available online at the publisher's website: ACS, https://doi.org/10.1021/acs.analchem.3c01982
La versión registrada de este artículo, publicado por primera vez en “Analytical Chemistry 2023, 95, 33, 12391–12390", está disponible en línea en el sitio web del editor: ACS, https://doi.org/10.1021/acs.analchem.3c01982
Categorías UNESCO
Palabras clave
Color, Colorimetry, Iron Serum, Testing and assessment
Citación
Dortez, S., DeGregorio-Rocasolano, N., Millán, M., GasullAgustín, T., G. Crevillen, A., Escarpa, A.(2023).Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients. Analytical Chemistry 2023, 95, 33, 12391–12397. https://doi.org/10.1021/acs.analchem.3c01982
Centro
Facultad de Ciencias
Departamento
Ciencias Analíticas