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A MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin serum samples

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dc.contributor.authorDurand Alegría, Jesús Senén
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorFernández Hernando, Pilar
dc.date.accessioned2024-05-20T11:18:24Z
dc.date.available2024-05-20T11:18:24Z
dc.date.issued2009-01-09
dc.description.abstractThis work reports a comparative study of two automated flow-through fluorosensors for the determination of digoxin in serum samples: an immunosensor with an anti-digoxin polyclonal antibody as the reactive phase permanently immobilized on CPG, and a sensor with a selective reaction system based on a methacrylic molecularly imprinted polymer (MIP) synthesized by bulk polymerisation. The variables affecting the sensitivity and dynamic range of the sensors (e.g., the carrier and elution solutions, flow-rates, pH and reagent concentrations) were optimised and the binding characteristics of their reactive phases compared in a competitive fluorescent assay. Digoxin was reproducibly determined by both sensors at the mg L-1 level (detection limit= 1.20×10-3 mg L-1 and RSD= 4-7% for the immunosensor; detection limit= 1.7×10-5 mg L-1 and RSD= 1-2% for the MIP sensor). No cross reactivity with digoxinrelated compounds was seen for either sensor at a digoxin:interferent ratio of 1:100. The lifetime of the immunosensor was about 50 immunoassays; its shelf life, when unused, is about three months. The lifetime of the MIP sensor was over 18 months. Both sensors were used to determine the digoxin concentration of human serum samples with satisfactory results.en
dc.description.versionversión final
dc.identifier.doihttps://doi.org/10.1007/s00216-008-2584-9
dc.identifier.issn1618-2642 - eISSN 1618-2650
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11609
dc.journal.titleAnalytical and Bioanalytical Chemistry
dc.journal.volume394
dc.language.isoen
dc.publisherSpringer
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject.keywordsDigoxin
dc.subject.keywordsPermanent immobilization
dc.subject.keywordsMolecular imprinting
dc.subject.keywordsFlow-through fluorosensor
dc.subject.keywordsSerum analysis
dc.titleA MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin serum sampleses
dc.typejournal articleen
dc.typeartículoes
dspace.entity.typePublication
relation.isAuthorOfPublication6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublicationf7c40fc3-8aca-42ef-a900-161d1b1be92e
relation.isAuthorOfPublication.latestForDiscovery6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
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