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Transferrin analysis in wistar rats plasma: Towards an electrochemical point-of-care approach for the screening of alcohol abuse

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dc.contributor.authorRodriguez-Hidalgo, Gema
dc.contributor.authorSierra, Tania
dc.contributor.authorDortez, Silvia
dc.contributor.authorMarcos, Alberto
dc.contributor.authorAmbrosio, Emilio
dc.contributor.authorGonzález Crevillén, Agustín
dc.contributor.authorEscarpa, Alberto
dc.date.accessioned2025-10-20T14:47:27Z
dc.date.available2025-10-20T14:47:27Z
dc.date.issued2022-06-29
dc.descriptionThe registered version of this article, first published in “Microchemical Journal,Vol 181,2022,10773.", is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.microc.2022.107738. La versión registrada de este artículo, publicado por primera vez en “Microchemical Journal,Vol 181,2022,10773.", está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.microc.2022.107738.
dc.description.abstractSimple and low-cost point-of-care testing (POCT) approaches are necessary not only for the diagnosis of alcohol abuse but also for the follow-up of patients during the treatment of alcohol dependence. To advance the development towards these approaches, a disposable screen printed-based electrochemical sensor is proposed for the diagnosis of chronic alcohol abuse. The selected biomarker to be screened (and followed up) was the carbohydrate deficient transferrin (CDT), which was assessed using the parameter “Electrochemical index of glycosylation” (EIG). EIG is defined as the ratio between the signal generated by carbohydrates present in transferrin (Tf) (selectively labeled with an Os(VI) tag) and the signal generated by the electroactive amino acids present in Tf, measured by adsorptive transfer square wave voltammetry (AdTSWV). The electrochemical sensor exhibited a good limit of detection (LOD ≤ 0.7 g L−1) well below the normal range of transferrin in serum (ranging from 2 to 3.6 g L−1). In addition, the diagnostic capabilities of the sensor were evaluated in plasma rats from 31 individuals (16 chronically exposed to alcohol and 15 saline control), yielding a very good clinical sensitivity and specificity (81% and 87%, respectively). These results draw the potential of this approach to be used for POCT of alcohol abuse, making it a useful tool to help therapists/clinicians even patient’s family in the follow-up of alcohol rehabilitation.en
dc.description.versionversión publicada
dc.identifier.citationRodriguez-Hidalgo,G., Sierra,T., Dortez,S., Marcos,A.,Ambrosio,E., G. Crevillen,A., Escarpa,A.(2022). Transferrin analysis in wistar rats plasma: Towards an electrochemical point-of-care approach for the screening of alcohol abuse, Microchemical Journal,Vol 181,2022,107738,https://doi.org/10.1016/j.microc.2022.107738.
dc.identifier.doihttps://doi.org/10.1016/j.microc.2022.107738
dc.identifier.issnISSN 0026-265X
dc.identifier.urihttps://hdl.handle.net/20.500.14468/30533
dc.journal.titleMicrochemical Journal
dc.journal.volume181
dc.language.isoen
dc.page.initial10773
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject2301 Química analítica
dc.subject6113.01 Alcoholismo
dc.subject.keywordsAlcoholism screeningen
dc.subject.keywordsDiagnosisen
dc.subject.keywordsPoint-of-care testingen
dc.subject.keywordsDisposable electrodesen
dc.subject.keywordsAnimal model testingen
dc.subject.keywordsRat plasmaen
dc.titleTransferrin analysis in wistar rats plasma: Towards an electrochemical point-of-care approach for the screening of alcohol abuseen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication0f39284a-d95a-443a-bb2e-21297ec40b76
relation.isAuthorOfPublication.latestForDiscovery0f39284a-d95a-443a-bb2e-21297ec40b76
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