Fecha
2022-06-29
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Derechos de acceso
info:eu-repo/semantics/openAccess
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Elsevier
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Resumen
Simple and low-cost point-of-care testing (POCT) approaches are necessary not only for the diagnosis of alcohol abuse but also for the follow-up of patients during the treatment of alcohol dependence.
To advance the development towards these approaches, a disposable screen printed-based electrochemical sensor is proposed for the diagnosis of chronic alcohol abuse. The selected biomarker to be screened (and followed up) was the carbohydrate deficient transferrin (CDT), which was assessed using the parameter “Electrochemical index of glycosylation” (EIG). EIG is defined as the ratio between the signal generated by carbohydrates present in transferrin (Tf) (selectively labeled with an Os(VI) tag) and the signal generated by the electroactive amino acids present in Tf, measured by adsorptive transfer square wave voltammetry (AdTSWV). The electrochemical sensor exhibited a good limit of detection (LOD ≤ 0.7 g L−1) well below the normal range of transferrin in serum (ranging from 2 to 3.6 g L−1). In addition, the diagnostic capabilities of the sensor were evaluated in plasma rats from 31 individuals (16 chronically exposed to alcohol and 15 saline control), yielding a very good clinical sensitivity and specificity (81% and 87%, respectively).
These results draw the potential of this approach to be used for POCT of alcohol abuse, making it a useful tool to help therapists/clinicians even patient’s family in the follow-up of alcohol rehabilitation.
Descripción
The registered version of this article, first published in “Microchemical Journal,Vol 181,2022,10773.", is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.microc.2022.107738.
La versión registrada de este artículo, publicado por primera vez en “Microchemical Journal,Vol 181,2022,10773.", está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.microc.2022.107738.
Categorías UNESCO
Palabras clave
Alcoholism screening, Diagnosis, Point-of-care testing, Disposable electrodes, Animal model testing, Rat plasma
Citación
Rodriguez-Hidalgo,G., Sierra,T., Dortez,S., Marcos,A.,Ambrosio,E., G. Crevillen,A., Escarpa,A.(2022). Transferrin analysis in wistar rats plasma: Towards an electrochemical point-of-care approach for the screening of alcohol abuse, Microchemical Journal,Vol 181,2022,107738,https://doi.org/10.1016/j.microc.2022.107738.
Centro
Facultad de Ciencias
Departamento
Ciencias Analíticas