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Occurrence of erythromycin residues in sheep milk. Validation of an analytical method

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dc.contributor.authorSoledad Rodríguez, Beatriz Elena
dc.contributor.authorDurand Alegría, Jesús Senén
dc.contributor.authorGarcía Mayor, M Asunción
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorFernández Hernando, Pilar
dc.contributor.authorGarcinuño Martínez, Rosa Mª
dc.date.accessioned2024-05-20T11:18:19Z
dc.date.available2024-05-20T11:18:19Z
dc.date.issued2015-04
dc.description.abstractThe paper describes a new and selective analytical sample treatment for quantitative extraction and preconcentration of erythromycin in presence of other macrolide antibiotics in sheep milk samples. The methodology is based on the use of a molecular imprinted polymer (MIP) employed as solid phase extraction sorbent (MISPE). The synthesized material by bulk polymerization using erythromycin (ERY) as template was evaluated as solid phase extraction sorbent, in a novel sample treatment technique that can be coupled to high-performance liquid chromatography with diode-array detector (HPLC-DAD). MIP selectivity was studied for other macrolide antibiotics with similar structures, such as tylosin (TYL), spiramycin (SPI), josamycin (JOS), roxithromycin (ROX) and ivermectin (IVER) getting recoveries for these interferents lower than 35%, for all cases except for ROX, which recoveries were around 85%. The variables affecting the molecularly imprinted solid-phase extraction (MISPE) procedure were optimized to select the best conditions of selectivity and sensitivity to determine ERY at concentration levels established by EU legislation in sheep milk. Under the selected experimental conditions, quantification limit was 24.1 µg kg−1. Recoveries were higher than 98%, with RSDs between 0.7% and 2%. The proposed MISPE-HPLC method was validated and successfully applied to ERY analysis in sheep milk samples.en
dc.description.versionversión final
dc.identifier.doihttps://doi.org/10.1016/j.fct.2014.12.020
dc.identifier.issn0278-6915 - eISSN: 1873-6351
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11603
dc.journal.titleFood and Chemical Toxicology
dc.journal.volume78
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject.keywordsErythromycin
dc.subject.keywordsMolecularly imprinted solid-phase extraction
dc.subject.keywordsSheep milk
dc.subject.keywordsHPLC-DAD
dc.titleOccurrence of erythromycin residues in sheep milk. Validation of an analytical methodes
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery5e470775-fe11-4279-be7d-7d42172f5413
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