Publicación: Neonatal inhibition of androgen activity alters the programming of body weight and orexinergic peptides differentially in male and female rats
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2024-02-13
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info:eu-repo/semantics/openAccess
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Elsevier
Resumen
The involvement of androgens in the regulation of energy metabolism has been demonstrated. The main
objective of the present research was to study the involvement of androgens in both the programming of energy
metabolism and the regulatory peptides associated with feeding. For this purpose, androgen receptors and the
main metabolic pathways of testosterone were inhibited during the first five days of postnatal life in male and
female Wistar rats. Pups received a daily s.c. injection from the day of birth, postnatal day (P) 1, to P5 of Flu-
tamide (a competitive inhibitor of androgen receptors), Letrozole (an aromatase inhibitor), Finasteride (a 5-
alpha-reductase inhibitor) or vehicle. Body weight, food intake and fat pads were measured. Moreover, hypo-
thalamic Agouti-related peptide (AgRP), neuropeptide Y (NPY), orexin, and proopiomelanocortin (POMC) were
analyzed by quantitative real-time polymerase chain reaction assay. The inhibition of androgenic activity during
the first five days of life produced a significant decrease in body weight in females at P90 but did not affect this
parameter in males. Moreover, the inhibition of aromatase decreased hypothalamic AgRP mRNA levels in males
while the inhibition of 5α-reductase decreased hypothalamic AgRP and orexin mRNA levels in female rats.
Finally, food intake and visceral fat, but not subcutaneous fat, were affected in both males and females depending
on which testosterone metabolic pathway was inhibited. Our results highlight the differential involvement of
androgens in the programming of energy metabolism as well as the AgRP and orexin systems during development
in male and female rats
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Palabras clave
Androgens, Programming, Orexigenic peptides, Sex differences, Rat
Citación
Centro
Facultades y escuelas::Facultad de Psicología
Departamento
Psicobiología