Publicación: Unveiling Molecular Effects of the Secondary Metabolite 2-Dodecanone in the Model Hymenopteran Nasonia vitripennis
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2024
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info:eu-repo/semantics/openAccess
Licencia Creative Commons
http://creativecommons.org/licenses/by/4.0/deed.es
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MDPI
Resumen
Over the past decade, multiple studies have suggested that the secondary metabolites produced by plants against herbivorous insects could be used as biopesticides. However, as the molecular mechanism of action of these compounds remains unknown, it is difficult to predict how they would affect non-target insects; thus, their innocuity needs to be clarified. Here, we investigate, from the molecular level to the organism, the responses of a useful parasitic insect Nasonia vitripennis (Walker, 1836) being exposed at the pupae stage for 48 h (up to 6 days) to sublethal doses (5 µg/L and 500 µg/L) of 2-Dodecanone. 2-Dodecanone altered the gene expression of genes related to ecdysone-related pathways, biotransformation, and cell homeostasis. A significant induction of ecdysone response-genes (EcR, usp, E78, Hr4, Hr38) was detected, despite no significant differences in ecdysteroid levels. Regarding the cell homeostasis processes, the gene l(2)efl was differentially altered in both experimental conditions, and a dose-dependent induction of hex81 was observed. 2-Dodecanone also triggered an induction of Cyp6aQ5 activity. Finally, 2-Dodecanone exposure had a significant effect on neither development time, energy reserves, nor egg-laying capacity; no potential genotoxicity was detected. For the first time, this study shows evidence that 2-Dodecanone can modulate gene expression and interfere with the ecdysone signalling pathway in N. vitripennis. This could lead to potential endocrine alterations and highlight the suitability of this organism to improve our general understanding of the molecular effects of plant defences in insects. Our findings provide new insights into the toxicity of 2-Dodecanone that could potentially be explored in other species and under field conditions for plant protection and pest management as a means to reduce reliance on synthetic pesticides.
Descripción
The registered version of this article, first published in “Toxics, vol. 12, 2024", is available online at the publisher's website: MDPI, https://doi.org/10.3390/toxics12020159
La versión registrada de este artículo, publicado por primera vez en “Toxics, vol. 12, 2024", está disponible en línea en el sitio web del editor: MDPI, https://doi.org/10.3390/toxics12020159
Categorías UNESCO
Palabras clave
methyl ketones, parasitoid wasp, toxicity assays, transcriptional alterations, physiological responses, reproduction
Citación
Planelló, R., Aquilino, M., Beaugeard, L., Llorente, L., Herrero, Ó., Siaussat, D., & Lécureuil, C. (2024). Unveiling Molecular Effects of the Secondary Metabolite 2-Dodecanone in the Model Hymenopteran Nasonia vitripennis. Toxics, 12(2), 159. https://doi.org/10.3390/toxics12020159
Centro
Facultad de Ciencias
Departamento
Física Matemática y de Fluídos