Publicación:
Unveiling Molecular Effects of the Secondary Metabolite 2-Dodecanone in the Model Hymenopteran Nasonia vitripennis

Vista sencilla de ítem
dc.contributor.authorPlanello Carro, María del Rosario
dc.contributor.authorAquilino , Mónica
dc.contributor.authorBeaugeard, Laureen
dc.contributor.authorLlorente Ortega, Lola
dc.contributor.authorHerrero Felipe, Óscar
dc.contributor.authorSiaussat, David
dc.contributor.authorLécureuil, Charlotte
dc.contributor.orcidhttps://orcid.org/0000-0002-1716-6889
dc.contributor.orcidhttps://orcid.org/0000-0003-2817-8997
dc.contributor.orcidhttps://orcid.org/0000-0002-6548-0370
dc.contributor.orcidhttps://orcid.org/0000-0002-7339-9185
dc.date.accessioned2025-01-27T12:45:31Z
dc.date.available2025-01-27T12:45:31Z
dc.date.issued2024
dc.descriptionThe registered version of this article, first published in “Toxics, vol. 12, 2024", is available online at the publisher's website: MDPI, https://doi.org/10.3390/toxics12020159 La versión registrada de este artículo, publicado por primera vez en “Toxics, vol. 12, 2024", está disponible en línea en el sitio web del editor: MDPI, https://doi.org/10.3390/toxics12020159
dc.description.abstractOver the past decade, multiple studies have suggested that the secondary metabolites produced by plants against herbivorous insects could be used as biopesticides. However, as the molecular mechanism of action of these compounds remains unknown, it is difficult to predict how they would affect non-target insects; thus, their innocuity needs to be clarified. Here, we investigate, from the molecular level to the organism, the responses of a useful parasitic insect Nasonia vitripennis (Walker, 1836) being exposed at the pupae stage for 48 h (up to 6 days) to sublethal doses (5 µg/L and 500 µg/L) of 2-Dodecanone. 2-Dodecanone altered the gene expression of genes related to ecdysone-related pathways, biotransformation, and cell homeostasis. A significant induction of ecdysone response-genes (EcR, usp, E78, Hr4, Hr38) was detected, despite no significant differences in ecdysteroid levels. Regarding the cell homeostasis processes, the gene l(2)efl was differentially altered in both experimental conditions, and a dose-dependent induction of hex81 was observed. 2-Dodecanone also triggered an induction of Cyp6aQ5 activity. Finally, 2-Dodecanone exposure had a significant effect on neither development time, energy reserves, nor egg-laying capacity; no potential genotoxicity was detected. For the first time, this study shows evidence that 2-Dodecanone can modulate gene expression and interfere with the ecdysone signalling pathway in N. vitripennis. This could lead to potential endocrine alterations and highlight the suitability of this organism to improve our general understanding of the molecular effects of plant defences in insects. Our findings provide new insights into the toxicity of 2-Dodecanone that could potentially be explored in other species and under field conditions for plant protection and pest management as a means to reduce reliance on synthetic pesticides.en
dc.description.versionversión publicada
dc.identifier.citationPlanelló, R., Aquilino, M., Beaugeard, L., Llorente, L., Herrero, Ó., Siaussat, D., & Lécureuil, C. (2024). Unveiling Molecular Effects of the Secondary Metabolite 2-Dodecanone in the Model Hymenopteran Nasonia vitripennis. Toxics, 12(2), 159. https://doi.org/10.3390/toxics12020159
dc.identifier.doihttps://doi.org/10.3390/toxics12020159
dc.identifier.issn2305-6304
dc.identifier.urihttps://hdl.handle.net/20.500.14468/25538
dc.journal.issue2
dc.journal.titleToxics
dc.journal.volume12
dc.language.isoen
dc.publisherMDPI
dc.relation.centerFacultad de Ciencias
dc.relation.departmentFísica Matemática y de Fluídos
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.es
dc.subject12 Matemáticas::1299 Otras especialidades matemáticas
dc.subject.keywordsmethyl ketonesen
dc.subject.keywordsparasitoid waspen
dc.subject.keywordstoxicity assaysen
dc.subject.keywordstranscriptional alterationsen
dc.subject.keywordsphysiological responsesen
dc.subject.keywordsreproductionen
dc.titleUnveiling Molecular Effects of the Secondary Metabolite 2-Dodecanone in the Model Hymenopteran Nasonia vitripennisen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
person.familyNamePlanello Carro
person.familyNameAquilino
person.familyNameHerrero Felipe
person.givenNameMaría del Rosario
person.givenNameMónica
person.givenNameÓscar
person.identifier.orcid0000-0003-3730-9549
person.identifier.orcid0000-0003-2817-8997
person.identifier.orcid0000-0002-1410-368X
relation.isAuthorOfPublication1de7b6f9-dd97-4518-8f0e-3c54768bfe6a
relation.isAuthorOfPublication910b4261-d72a-4bf6-9e59-a0df144771b0
relation.isAuthorOfPublication16da09a8-34db-4259-90d7-36928325faff
relation.isAuthorOfPublication.latestForDiscovery1de7b6f9-dd97-4518-8f0e-3c54768bfe6a
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
HerreroFelipe_Oscar_2-dodecanoneNasonia.pdf
Tamaño:
2.03 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
3.62 KB
Formato:
Item-specific license agreed to upon submission
Descripción:
Descargar
Colecciones
Artículos y papers