Publicación: Intergenerational Patterns of DNA Methylation in Procambarus clarkii Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous?
Fecha
2021
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Derechos de acceso
info:eu-repo/semantics/openAccess
Título de la revista
ISSN de la revista
Título del volumen
Editor
MDPI
Resumen
Epigenome is susceptible to modulation by environmental pressures—namely, through alterations in global DNA methylation, impacting the organism condition and, ultimately, reverberating on the phenotype of the subsequent generations. Hence, an intergenerational study was conducted, aiming to clarify the influence of genotoxicants on global DNA methylation of the crayfish Procambarus clarkii. Two subsequent generations were exposed to the herbicide penoxsulam (Px; 23 µg·L−1) and to the genotoxicant model ethyl methanesulfonate (EMS; 5 mg·L−1). Px did not induce changes in DNA methylation of adult crayfish (F0). However, the hypomethylation occurring in unexposed F1 juveniles demonstrated that the history of exposure per se can modulate epigenome. In F1 descendants of the Px-exposed group, methylome (hypermethylated) was more affected in males than in females. EMS-induced hypomethylation in adult females (F0), also showed gender specificity. In addition, hypomethylation was also observed in the unexposed F1 crayfish, indicating an intergenerational epigenetic effect. The modulatory role of past exposure to penoxsulam or to EMS also showed a dependency on the crayfish developmental stage. Overall, this research revealed that indirect experiences (events occurring in a predecessor generation) can have an impact even greater than direct experiences (present events) on the epigenetic dynamics.
Descripción
The registered version of this article, first published in “Toxics, vol. 9, 2021", is available online at the publisher's website: MDPI, https://doi.org/10.3390/toxics9110271
La versión registrada de este artículo, publicado por primera vez en “Toxics, vol. 9, 2021", está disponible en línea en el sitio web del editor: MDPI, https://doi.org/10.3390/toxics9110271
Categorías UNESCO
Palabras clave
crustacean, epigenetics, methylome, intergenerational, genotoxic, pesticides, penoxsulam, epigenotoxicology
Citación
Marçal, R., Llorente, L., Herrero, O., Planelló, R., Guilherme, S., & Pacheco, M. (2021). Intergenerational Patterns of DNA Methylation in Procambarus clarkii Following Exposure to Genotoxicants: A Conjugation in Past Simple or Past Continuous? Toxics, 9(11), 271. https://doi.org/10.3390/toxics9110271
Centro
Facultades y escuelas::Facultad de Ciencias
Departamento
Física Matemática y de Fluídos