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Fibreglass membrane chemically modified with amino-functionalised SBA-15 and its application in solid-phase extraction to determine macrolide antibiotics in eggs

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dc.contributor.authorGonzález Gómez, Lorena
dc.contributor.authorMorante Zarcero, Sonia
dc.contributor.authorPérez Quintanilla, Damián
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorGarcinuño Martínez, Rosa Mª
dc.contributor.authorFernández Hernando, Pilar
dc.contributor.authorSierra, Isabel
dc.date.accessioned2024-07-31T07:39:42Z
dc.date.available2024-07-31T07:39:42Z
dc.date.issued2024-10
dc.description.abstractMacrolides are a group of high-spectrum antibiotics used to treat animal ailments, sometimes these drugs can appear as residues in animal foods such as eggs, which must be controlled as required by the European Union. Therefore, in this paper a methodology based on solid–liquid extraction followed on the application of a new membrane-solid-phase extraction (membrane-SPE) was developed and validated for the extraction and purification of five macrolide antibiotics such as spiramycin (SPI), erythromycin (ERY), roxithromycin (ROX), josamycin (JOS) and tylosin (TYL), in egg samples for their analysis by high-performance liquid chromatography coupled to mass spectrometry analysis (HPLC-MS/MS). The newly synthesised membrane-based sorbent consisted of a fibreglass membrane chemically modified with silica SBA-15 type functionalised with amino groups (FGM-SBA-15-NH2). The optimal conditions for membrane-SPE on eggs were determined using a three-factor, three-level Box Behnken design (BBD). The conditions obtained were by loading with 4 mL of water and eluting with 3 × 2.5 mL of MeOH with ammonia solution (0.5 %, v/v). The methodology was validated according to Regulation (EU) 2021/808 showing good selectivity, linearity (R2 ≥ 0.993), low limits (MQL 1.1–2.1 ng/g, MDL 0.3–0.6 ng/g), acceptable decision limit for confirmation (CCα), good accuracy (recoveries 85–100 %) and an adequate precision (RSD ≤ 16 %). The FGM-SBA-15-NH2 proved to be reproducible in different batches and reusable around 70 times. Finally, the validated method was successfully applied to fourteen egg samples detecting SPI in one hen egg sample and JOS and ROX in one quail egg sample. The proposed methodology introduces a groundbreaking membrane-SPE approach, addressing limitations in conventional SPE methods. The use of functionalised silica to chemically modify FGM significantly improves its textural properties, allowing the simultaneous extraction and purification of macrolide antibiotics from egg samples.en
dc.description.versionversión publicada
dc.identifier.citationLorena González-Gómez, Sonia Morante-Zarcero, Damián Pérez-Quintanilla, Gema Paniagua González, Rosa M. Garcinuño, Pilar Fernández Hernando, Isabel Sierra, Fibreglass membrane chemically modified with amino-functionalised SBA-15 and its application in solid-phase extraction to determine macrolide antibiotics in eggs, Microchemical Journal, Volume 205, 2024, 111232, , https://doi.org/10.1016/j.microc.2024.111232.
dc.identifier.doihttps://doi.org/10.1016/j.microc.2024.111232
dc.identifier.issn0026-265X
dc.identifier.urihttps://hdl.handle.net/20.500.14468/23164
dc.journal.titleMicrochemical Journal
dc.journal.volume205
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultades y escuelas::Facultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/deed.es
dc.subject23 Química
dc.subject.keywordsMacrolide antibioticsen
dc.subject.keywordsEggsen
dc.subject.keywordsFibreglass membraneen
dc.subject.keywordsAmino-silica membraneen
dc.subject.keywordsMembrane-solid-phase extractionen
dc.subject.keywordsLiquid chromatography-mass spectrometryen
dc.titleFibreglass membrane chemically modified with amino-functionalised SBA-15 and its application in solid-phase extraction to determine macrolide antibiotics in eggsen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublication.latestForDiscovery6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
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