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Gene expression analysis of Chironomus riparius in response to acute exposure to tire rubber microparticles and leachates

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dc.contributor.authorCaballero Carretero, Patricia
dc.contributor.authorCarrasco Navarro, V.
dc.contributor.authorKukkonen, J.V.K.
dc.contributor.authorMartínez Guitarte, José Luis
dc.date.accessioned2024-10-07T12:14:25Z
dc.date.available2024-10-07T12:14:25Z
dc.date.issued2023-12-08
dc.description.abstractTire rubber microparticles (TRPs) entering aquatic ecosystems through stormwater runoffs is a significant challenge. TRPs are formed by the abrasion of tires with the road surface and include chemical additives that are an additional cause for concern. Currently, information on the molecular effects of TRPs, or especially its additives, in freshwater organisms is scarce. To address this problem, an array covering different cellular processes has been designed for the freshwater midge Chironomus riparius. Fourth-instar larvae were exposed to two concentrations of TRPs (1 mg L 1, 10 mg L 1) and tire rubber leachates (TRLs) (0,0125 %, 5 %) to evaluate the transcriptional activity by Real-Time PCR. To assess acute toxicity, larvae were exposed for 24 h and genes related to the endocrine system, stress response, DNA repair mechanisms, immune system, oxidative stress, and detoxification mechanisms were evaluated. The activity of the enzymes: glutathione S-transferase (GST) and catalase was also examined. The main pathway affected was the stress response showing overexpression of HSPs (HSC70.3, HSC70.4, HSC70.5, HSP60). Moreover, there was a reduction of the GSTd3 and catalase disrupting the antioxidant system. The upregulation of InR indicates a potential disturbance in the insulin pathway and ABCB6 activation only in TRPs exposure suggests its potential implication in their transport. However, most of these alterations are caused by TRLs, showing higher toxicity than TRPs. The results obtained in this work provide the first approach at the molecular and cellular levels to elucidate the impact of TRLs in freshwater organisms. To perform a realistic evaluation of the TR effects, additional research is required to assess the TR’s long-term effects at the molecular level.en
dc.description.versionversión publicada
dc.identifier.citationP. Caballero-Carretero, V. Carrasco-Navarro, J.V.K. Kukkonen, J.L. Martínez-Guitarte, Gene expression analysis of Chironomus riparius in response to acute exposure to tire rubber microparticles and leachates, Environmental Pollution, Volume 342, 2024, 123111, ISSN 0269-7491, https://doi.org/10.1016/j.envpol.2023.123111
dc.identifier.doihttps://doi.org/10.1016/j.envpol.2023.123111
dc.identifier.issn0269-7491
dc.identifier.urihttps://hdl.handle.net/20.500.14468/23940
dc.journal.titleEnvironmental Pollution
dc.journal.volume342
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultades y escuelas::Facultad de Ciencias
dc.relation.departmentFísica Matemática y de Fluídos
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.es
dc.subject33 Ciencias Tecnológicas
dc.subject.keywordsTire rubberen
dc.subject.keywordsParticlesen
dc.subject.keywordsLeachatesen
dc.subject.keywordsGene expressionen
dc.subject.keywordsStressen
dc.titleGene expression analysis of Chironomus riparius in response to acute exposure to tire rubber microparticles and leachatesen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication4719b68d-d887-4215-9155-950f84aabed5
relation.isAuthorOfPublicationa078395f-9ae2-4e6a-b18c-2549d07bdea9
relation.isAuthorOfPublication.latestForDiscovery4719b68d-d887-4215-9155-950f84aabed5
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