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On-line flow injection molecularly imprinted solid phase extraction for the preconcentration and determination of 1-hydroxypyrene in urine samples

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dc.contributor.authorSerrano, Montserrat
dc.contributor.authorBartolomé, Mónica
dc.contributor.authorGañán Aceituno, Judith
dc.contributor.authorGallego Picó, Alejandrina
dc.contributor.authorBravo Yagüe, Juan Carlos
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorGarcinuño Martínez, Rosa Mª
dc.date.accessioned2024-05-20T11:18:02Z
dc.date.available2024-05-20T11:18:02Z
dc.date.issued2017-05-01
dc.description.abstractNew analytical strategies tend to automation of sample pre-treatment and flow analysis techniques provided a number of enhanced analytical methods allowing high throughput. Flow techniques are usually faster, more robust and more flexible than their batch equivalents. In addition, flow methods use less sample and reagent amounts and reduce analytical costs and waste. A flow injection solid-phase extraction pre-concentration system using a molecularly imprinted polymer (MIP) packed micro-column was developed for the determination of 1-hydroxypyrene in human urine with fluorescence detection. The pre-concentration of 1-hydroxypyrene on the MIP was carried out based on the specific retention of analyte by on-line introducing the sample into the micro-column system. Methanol and dichloromethane mixture was used to elute the retained analyte for fluorometric analysis. Important influencing factors were studied in detail, in batch and in flow (MISPE procedure optimisation, sample and eluent volumes, flow rate, dimensions of MIP micro-column and amounts of packing material, etc). To the best of our knowledge, this is the first on-line flow injection molecularly imprinted solid phase extraction for the pre-concentration and determination of hydroxylate PAH metabolite in urine samples. The optimised method was successfully applied to the determination of 1-Hydroxypyrene in spiked urine samples, with recoveries in the range of 74–85% and RSD<4.6%. Under optimum experimental conditions, the linearity concentration range used was 10–400 μg L−1, R2>0.996. We obtained limit of detection and quantification of 3.1 μg L−1 and 10.5 μg L−1, respectivelyen
dc.description.versionversión publicada
dc.identifier.doihttps://doi.org/10.1016/j.talanta.2016.01.048
dc.identifier.issn0039-9140
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11583
dc.journal.titleTalanta
dc.journal.volume166
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject.keywords1-Hydroxypyrene
dc.subject.keywordsOn-line solid-phase extraction
dc.subject.keywordsMolecularly imprinted polymer
dc.subject.keywordsHuman urine
dc.subject.keywordsFlow injection analysis
dc.titleOn-line flow injection molecularly imprinted solid phase extraction for the preconcentration and determination of 1-hydroxypyrene in urine sampleses
dc.typejournal articleen
dc.typeartículoes
dspace.entity.typePublication
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relation.isAuthorOfPublication6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublicationdb6e39ea-d23e-4b0b-94ba-0cd2c62308fb
relation.isAuthorOfPublication.latestForDiscovery6eea5c71-1f97-4dcb-93ef-9e1cbc1e7f46
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