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A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes

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dc.contributor.authorDortez, Silvia
dc.contributor.authorPacheco, Marta
dc.contributor.authorGasull, Teresa
dc.contributor.authorGonzález Crevillén, Agustín
dc.contributor.authorEscarpa, Alberto
dc.date.accessioned2025-10-21T11:38:17Z
dc.date.available2025-10-21T11:38:17Z
dc.date.issued2024-07-29
dc.descriptionThe registered version of this article, first published in “Lab on a Chip, 24: 4253-4263", is available online at the publisher's website: The Royal Society of Chemistry, https://doi.org/10.1039/D4LC00398E La versión registrada de este artículo, publicado por primera vez en “Lab on a Chip, 24: 4253-4263", está disponible en línea en el sitio web del editor: The Royal Society of Chemistry, https://doi.org/10.1039/D4LC00398E
dc.description.abstractA novel microfluidic paper-based analytical device with dual colorimetric and electrochemical detection (dual μPAD) was developed for the assessment of transferrin saturation (TSAT) in samples from ischemic stroke patients. TSAT was calculated from the ratio between transferrin-bound iron, which was colorimetrically measured, and the total iron-binding capacity, which was electrochemically measured. To this end, a μPAD was smartly designed, which integrated both colorimetric and electrochemical detection reservoirs, communicating via a microchannel acting as a chemical reactor, and with preloading/storing capabilities (reagent-free device). This approach allowed the dual and simultaneous determination of both parameters, providing an improvement in the reliability of the results due to an independent signal principle and processing. The μPADs were validated by analyzing a certified reference material, showing excellent accuracy (Er ≤ 5%) and precision (RSD ≤ 2%). Then they were applied to the analysis of diagnosed serum samples from ischemic stroke patients. The results were compared to those provided by a free-interference method (urea-PAGE). Impressively, both methods exhibited a good correlation (r = 0.96, p < 0.05) and no significant differences were found between them (slope 1.0 ± 0.1 and the intercept 1 ± 4, p < 0.05), demonstrating the excellent accuracy of our approach during the analysis of complex samples from ischemic stroke patients, using just 90 μL of clinical samples and taking less than 90 min in comparison with the 18 hours required by the urea-PAGE approach. The developed fully integrated colorimetric-electrochemical μPAD is a promising ready to use reagent-free device for the point-of-care testing of TSAT, which can be used to assist physicians in the fast diagnosis and prognosis of ischemic strokes, where the decision-time is crucial for the patient's survival.en
dc.description.versionversión publicada
dc.identifier.citationDortez, S., Pacheco, M., Gasull, T., G. Crevillen, A., Escarpa, A., (2024).A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes . Lab on a Chip, 24: 4253-4263. https://doi.org/10.1039/D4LC00398E
dc.identifier.doihttps://doi.org/10.1039/D4LC00398E
dc.identifier.issneISSN: 1473-0189
dc.identifier.urihttps://hdl.handle.net/20.500.14468/30557
dc.journal.issue24
dc.journal.titleLab on a Chip
dc.language.isoen
dc.page.final4263
dc.page.initial4253
dc.publisherThe Royal Society of Chemistry
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/deed.es
dc.subject2301 Química analítica
dc.subject3303.09 Operaciones electroquímicas
dc.subject.keywordsColorimetrices
dc.subject.keywordsElectrochemicales
dc.subject.keywordsMicrofluidices
dc.titleA dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokesen
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dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication0f39284a-d95a-443a-bb2e-21297ec40b76
relation.isAuthorOfPublication.latestForDiscovery0f39284a-d95a-443a-bb2e-21297ec40b76
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