Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer

Durand Alegría, Jesús Senén; Paniagua González, Gema; Fernández Hernando, Pilar

Publicación:
Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer

dc.contributor.author	Durand Alegría, Jesús Senén
dc.contributor.author	Paniagua González, Gema
dc.contributor.author	Fernández Hernando, Pilar
dc.date.accessioned	2024-05-20T11:18:27Z
dc.date.available	2024-05-20T11:18:27Z
dc.date.issued	2008-06-15
dc.description.abstract	This work describes the development of a competitive flow-through FIA assay for digoxin using a molecularly imprinted polymer (MIP) as the recognition phase. In previous work, a number of non-covalent imprinted polymers were synthesised by “bulk” polymerisation. The digoxin binding and elution characteristics of these MIPs were then evaluated to obtain a highly selective material for integration into a sensor. The optimum MIP was synthesised by photo-initiated polymerisation of a mixture containing digoxin, MAA, EDGMA and AIBN in acetonitrile. The bulk polymer was ground and sieved and the template removed by Soxhlet extraction in MeOH/ACN. The MIP was packed into a flow cell and placed in a spectrofluorimeter to integrate the reaction and detection systems. The physical and chemical variables involved in digoxin determination by the sensor (nature and concentration of solution, flow rates, etc.) were optimised. Binding with the non-imprinted polymer (NIP) was also analysed. The new fluorosensor showed high selectivity and sensitivity, a detection limit of 1.7 × 10−2 μg l−1, and high reproducibility (R.S.D. of 1.03% and 1.77% for concentrations of 1.0 × 10−3 and 4.0 × 10−3 mg l−1, respectively). Selectivity was tested by determining the cross-reactivity of several compounds with structures analogous to digoxin. Under the assay conditions used, in which the potential interfering compounds were in concentrations 100 times higher than that of the analyte, no interference was recorded. The proposed fluorosensor was successfully used to determine digoxin concentration of human serum samples.	en
dc.description.version	versión final
dc.identifier.doi	https://doi.org/10.1016/j.bios.2008.01.018
dc.identifier.issn	0956-5663 - eISSN 1873-4235
dc.identifier.uri	https://hdl.handle.net/20.500.14468/11612
dc.journal.issue	11
dc.journal.title	Biosensors and Bioelectronics
dc.journal.volume	23
dc.language.iso	en
dc.publisher	Elsevier
dc.relation.center	Facultad de Ciencias
dc.relation.department	Ciencias Analíticas
dc.rights	Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject.keywords	Digoxin
dc.subject.keywords	Molecular imprinting
dc.subject.keywords	Fluorosensor
dc.subject.keywords	Human serum analysis
dc.title	Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer	es
dc.type	artículo	es
dc.type	journal article	en
dspace.entity.type	Publication
relation.isAuthorOfPublication	6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublication	f7c40fc3-8aca-42ef-a900-161d1b1be92e
relation.isAuthorOfPublication.latestForDiscovery	6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11