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Determination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymer

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dc.contributor.authorDurand Alegría, Jesús Senén
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorFernández Hernando, Pilar
dc.date.accessioned2024-05-20T11:18:27Z
dc.date.available2024-05-20T11:18:27Z
dc.date.issued2008-06-15
dc.description.abstractThis work describes the development of a competitive flow-through FIA assay for digoxin using a molecularly imprinted polymer (MIP) as the recognition phase. In previous work, a number of non-covalent imprinted polymers were synthesised by “bulk” polymerisation. The digoxin binding and elution characteristics of these MIPs were then evaluated to obtain a highly selective material for integration into a sensor. The optimum MIP was synthesised by photo-initiated polymerisation of a mixture containing digoxin, MAA, EDGMA and AIBN in acetonitrile. The bulk polymer was ground and sieved and the template removed by Soxhlet extraction in MeOH/ACN. The MIP was packed into a flow cell and placed in a spectrofluorimeter to integrate the reaction and detection systems. The physical and chemical variables involved in digoxin determination by the sensor (nature and concentration of solution, flow rates, etc.) were optimised. Binding with the non-imprinted polymer (NIP) was also analysed. The new fluorosensor showed high selectivity and sensitivity, a detection limit of 1.7 × 10−2 μg l−1, and high reproducibility (R.S.D. of 1.03% and 1.77% for concentrations of 1.0 × 10−3 and 4.0 × 10−3 mg l−1, respectively). Selectivity was tested by determining the cross-reactivity of several compounds with structures analogous to digoxin. Under the assay conditions used, in which the potential interfering compounds were in concentrations 100 times higher than that of the analyte, no interference was recorded. The proposed fluorosensor was successfully used to determine digoxin concentration of human serum samples.en
dc.description.versionversión final
dc.identifier.doihttps://doi.org/10.1016/j.bios.2008.01.018
dc.identifier.issn0956-5663 - eISSN 1873-4235
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11612
dc.journal.issue11
dc.journal.titleBiosensors and Bioelectronics
dc.journal.volume23
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject.keywordsDigoxin
dc.subject.keywordsMolecular imprinting
dc.subject.keywordsFluorosensor
dc.subject.keywordsHuman serum analysis
dc.titleDetermination of digoxin in serum samples using a flow-through fluorosensor based on a molecularly imprinted polymeres
dc.typejournal articleen
dc.typeartículoes
dspace.entity.typePublication
relation.isAuthorOfPublication6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublicationf7c40fc3-8aca-42ef-a900-161d1b1be92e
relation.isAuthorOfPublication.latestForDiscovery6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
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