Publicación: Alteraciones metabólicas en la administración conjunta de alcohol y cocaína en ratas
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2018-07-03
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info:eu-repo/semantics/openAccess
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Universidad Nacional de Educación a Distancia (España). Facultad de Psicología. Departamento de Metodología de las Ciencias del Comportamiento y de la Salud
Resumen
Uno de los problemas de salud más graves que enfrentan actualmente los países desarrollados es el elevado consumo de alcohol. Su combinación con la cocaína es un patrón cada vez más extendido, especialmente entre los adolescentes. Cuando el alcohol y la cocaína se consumen juntas se produce el metabolito cocaetileno, cuya toxicidad es superior a la producida por el consumo individual de cada una de estas drogas. Es preciso el desarrollo de nuevos estudios enfocados a descubrir otros posibles metabolitos y/o rutas metabólicas que podrían estar alterados cuando ambas drogas se consumen de forma combinada. Este trabajo se desarrolla en el marco de un proyecto en el que se investigan modelos animales de administración pasiva y autoadministración de alcohol y cocaína. Se han utilizado técnicas de química analítica para detectar metabolitos que pudieran ser relevantes. Los resultados hasta el momento apuntan a la implicación de los aminoácidos. En el presente estudio se utilizaron 90 ratas Wistar de ambos sexos en la etapa de la adolescencia y de adulto joven. Los sujetos se asignaron aleatoriamente a cuatro grupos con administración intravenosa de las drogas: alcohol (2 g/kg-p.v.), cocaína (5 mg/kg-p.v.), alcohol + cocaína (2 g/kg-p.v. + 15 mg/kg-p.v.) y control salino (0,9% p/v de cloruro sódico). Mediante la técnica analítica de electroforesis capilar con fluorescencia inducida por láser (CE-LIF) y una metabolómica dirigida se midieron las concentraciones de 11 aminoácidos en el plasma sanguíneo de las ratas. El objetivo fue buscar una estructura más simple que permitiera explicar la variabilidad de estos aminoácidos en los grupos sometidos a diferentes tratamientos con las drogas. En un análisis factorial exploratorio (AFE) se extrajeron cuatro factores que explicaban el 62,55% de la varianza debida a los factores comunes entre las variables estudiadas. En sucesivos análisis de varianza factoriales (ANOVAs), con las puntuaciones de las ratas en cada dimensión como variable dependiente, se encontraron diferencias significativas en función del tratamiento recibido.
One of the most serious health problems facing developed countries today is the high consumption of alcohol. Its combination with cocaine is an increasingly widespread pattern, especially among adolescents. When alcohol and cocaine are consumed together, the metabolite cocaethylene is produced, whose toxicity is higher than that produced by consumption of each of these drugs alone. It is necessary to develop new studies focused on discovering other possible metabolites and/or metabolic pathways that could be altered when both drugs are consumed in combination. This work is developed within the framework of a project in which animal models of passive administration and self-administration of alcohol and cocaine are being investigated. Analytical chemistry techniques have been used to detect metabolites that may be relevant. The results so far point to the involvement of amino acids. In the present study 90 Wistar rats of both sexes were used in adolescence and young adult stages. The subjects were randomly assigned to four groups with intravenous drug administration: alcohol (2 g/kg-pv), cocaine (5 mg/kg-pv.), alcohol + cocaine (2 g/kg-pv + 15 mg / kg-pv) and saline control (0.9% w/v sodium chloride). By means of the analytical technique of capillary electrophoresis with laser-induced fluorescence (CE-LIF) and a directed metabolomics the concentrations of 11 amino acids in the blood plasma of the rats were measured. The objective was to find a simpler structure that would explain the variability of these amino acids in the groups subjected to different treatments with drugs. In an exploratory factorial analysis (AFE), four factors were extracted that explained 62.55% of the variance due to the common factors among the variables studied. In successive factorial analyzes of variance (ANOVAs), with the scores of the rats in each dimension as a dependent variable, significant differences were found depending on the treatment received.
One of the most serious health problems facing developed countries today is the high consumption of alcohol. Its combination with cocaine is an increasingly widespread pattern, especially among adolescents. When alcohol and cocaine are consumed together, the metabolite cocaethylene is produced, whose toxicity is higher than that produced by consumption of each of these drugs alone. It is necessary to develop new studies focused on discovering other possible metabolites and/or metabolic pathways that could be altered when both drugs are consumed in combination. This work is developed within the framework of a project in which animal models of passive administration and self-administration of alcohol and cocaine are being investigated. Analytical chemistry techniques have been used to detect metabolites that may be relevant. The results so far point to the involvement of amino acids. In the present study 90 Wistar rats of both sexes were used in adolescence and young adult stages. The subjects were randomly assigned to four groups with intravenous drug administration: alcohol (2 g/kg-pv), cocaine (5 mg/kg-pv.), alcohol + cocaine (2 g/kg-pv + 15 mg / kg-pv) and saline control (0.9% w/v sodium chloride). By means of the analytical technique of capillary electrophoresis with laser-induced fluorescence (CE-LIF) and a directed metabolomics the concentrations of 11 amino acids in the blood plasma of the rats were measured. The objective was to find a simpler structure that would explain the variability of these amino acids in the groups subjected to different treatments with drugs. In an exploratory factorial analysis (AFE), four factors were extracted that explained 62.55% of the variance due to the common factors among the variables studied. In successive factorial analyzes of variance (ANOVAs), with the scores of the rats in each dimension as a dependent variable, significant differences were found depending on the treatment received.
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alcohol, cocaína, metabolómica, modelos animales, análisis factorial exploratorio, AFE, cocaine, metabolomics, animal models, exploratory factor analysis, EFA
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Facultades y escuelas::Facultad de Psicología
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