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Measuring DNA modifications with the comet assay: a compendium of protocols

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dc.contributor.authorCollins, Andrew
dc.contributor.authorMøller, Peter
dc.contributor.authorGajsk, Goran
dc.contributor.authorVodenková, Soňa
dc.contributor.authorHerrero Felipe, Óscar
dc.contributor.authoret al
dc.date.accessioned2025-01-21T10:16:11Z
dc.date.available2025-01-21T10:16:11Z
dc.date.issued2023-01-27
dc.descriptionThis is an Accepted Manuscript of an article published by Nature Research in "Nature Protocols 18(3), 929-989", available at: https://doi.org/10.1038/s41596-022-00754-y
dc.descriptionEste es el manuscrito aceptado del artículo publicado por Nature Research en "Nature Protocols 18(3), 929-989", disponible en línea: https://doi.org/10.1038/s41596-022-00754-y
dc.description.abstractThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA–DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.en
dc.description.versionversión final
dc.identifier.citationCollins, A., Møller, P., Gajski, G. et al. Measuring DNA modifications with the comet assay: a compendium of protocols. Nat Protoc 18, 929–989 (2023). https://doi.org/10.1038/s41596-022-00754-y
dc.identifier.doihttps://doi.org/10.1038/s41596-022-00754-y
dc.identifier.issn1754-2189 | eISSN 1750-2799
dc.identifier.urihttps://hdl.handle.net/20.500.14468/25402
dc.journal.issue3
dc.journal.titleNature Protocols
dc.journal.volume18
dc.language.isoen
dc.page.final989
dc.page.initial929
dc.publisherNature Research
dc.relation.centerFacultades y escuelas::Facultad de Ciencias
dc.relation.departmentFísica Matemática y de Fluídos
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject22 Física
dc.titleMeasuring DNA modifications with the comet assay: a compendium of protocolsen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication16da09a8-34db-4259-90d7-36928325faff
relation.isAuthorOfPublication.latestForDiscovery16da09a8-34db-4259-90d7-36928325faff
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