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Selective extraction of diazepam and its metabolites from urine samples by a molecularly imprinted solid phase extraction (MISPE) method

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dc.contributor.authorGil Tejedor, Ana María
dc.contributor.authorBravo Yagüe, Juan Carlos
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorFernández Hernando, Pilar
dc.contributor.authorGarcinuño Martínez, Rosa Mª
dc.date.accessioned2024-05-20T11:18:16Z
dc.date.available2024-05-20T11:18:16Z
dc.date.issued2024-02
dc.description.abstractIn this research, a molecularly imprinted polymer (MIP) was synthesized by precipitation polymerization using oxazepam (OZ) as a template molecule and was subsequently applied as a selective sorbent for the extraction of diazepam (DZP) and its metabolites in urine samples using an SPE cartridge. OZ, temazepam (TZ), nordiazepam (NZ) and DZP were analyzed in the final extracts by high-performance liquid chromatography with diode array detection (HPLC-DAD). The SPE extraction steps were optimized, and the evaluation of an imprinting factor was carried out. The selectivity of the method for OZ versus structurally related benzodiazepines (BZDs), such as bromazepam (BRZ), tetrazepam (TTZ) and halazepam (HZ), was investigated. Under the optimum conditions, the proposed methodology provided good linearity in the range of 10–1500 ng/mL, with limit of detection values between 13.5 and 21.1 ng/mL and recovery levels for DZP and its metabolites from 89.0 to 93.9% (RSD ≤ 8%) at a concentration level of 1000 ng/mL. The proposed method exhibited good selectivity, precision and accuracy and was applied to the analysis of urine samples from a real case of DZP intake.en
dc.description.versionversión publicada
dc.identifier.doihttps://doi.org/10.3390/polym16050635
dc.identifier.issn2073-4360
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11600
dc.journal.issue5
dc.journal.titlePolymers
dc.journal.volume16
dc.language.isoen
dc.publisherMDPI
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsAtribución 4.0 Internacional
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0
dc.subject.keywordsbenzodiazepines
dc.subject.keywordsmolecular imprinting solid-phase extraction
dc.subject.keywordsurine samples
dc.subject.keywordsHPLC-DAD
dc.titleSelective extraction of diazepam and its metabolites from urine samples by a molecularly imprinted solid phase extraction (MISPE) methodes
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery6eea5c71-1f97-4dcb-93ef-9e1cbc1e7f46
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