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Prodiamesa olivacea: de novo biomarker genes in a potential sentinel organism for ecotoxicity studies in natural scenarios

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dc.contributor.authorLlorente, Lola
dc.contributor.authorHerrero Felipe, Óscar
dc.contributor.authorAquilino Amez, Mónica
dc.contributor.authorPlanello Carro, María del Rosario
dc.date.accessioned2025-01-22T09:52:47Z
dc.date.available2025-01-22T09:52:47Z
dc.date.issued2020-10
dc.descriptionThis is an Accepted Manuscript of an article published by Elsevier in "Aquatic Toxicology, 227, 105593", available at: https://doi.org/10.1016/j.aquatox.2020.105593
dc.descriptionEste es el manuscrito aceptado del artículo publicado por Elsevier en "Aquatic Toxicology, 227, 105593", disponible en línea en: https://doi.org/10.1016/j.aquatox.2020.105593
dc.description.abstractAlong with traditional ecotoxicological approaches in model organisms, toxicological studies in non-model organisms are being taken into consideration in order to complement them and contribute to more robust approaches. This allows us to figure out the complexity of the exposures involved in natural ecosystems. In this context, in the present research we have used the model species Chironomus riparius (Chironomidae, Diptera) and the non-model species Prodiamesa olivacea (Chironomidae, Diptera) to assess the aquatic toxic effects of acute 4-h and 24-h exposures to 1 μgL−1 of three common environmental pollutants: butyl benzyl phthalate (BBP), bisphenol A (BPA), and benzophenone 3 (BP3). Individuals of both species were collected from a contaminated river (Sar) in Galicia (Spain). Regarding Chironomus, there are four OECD standardized tests for the evaluation of water and sediment toxicity, in which different species in this genus can be used to assess classical toxicity parameters such as survival, immobilization, reproduction, and development. In contrast, Prodiamesa is rarely used in toxicity studies, even though it is an interesting toxicological species because it shares habitats with Chironomus but requires less extreme conditions (e.g., contamination) and higher oxygen levels. These different requirements are particularly interesting in assessing the different responses of both species to pollutant exposure. Quantitative real-time PCR was used to evaluate the transcriptional changes caused by xenobiotics in different genes of interest. Since information about P. olivacea in genomic databases is scarce, its transcriptome was obtained using de novo RNAseq. Genes involved in biotransformation pathways and the oxidative stress response (MnSOD, CAT, PHGPx, Cyp4g15, Cyp6a14-like and Cyp6a2-like) were de novo identified in this species. Our results show differential toxic responses depending on the species and the xenobiotic, being P. olivacea the dipteran that showed the most severe effects in most of the studied biomarker genes. This work represents a multi-species approach that allows us to deepen in the toxicity of BBP, BPA, and BP3 at the molecular level. Besides, it provides an assessment of the tolerance/sensitivity of natural populations of model and non-model insect species chronically exposed to complex mixtures of pollutants in natural scenarios. These findings may have important implications for understanding the adverse biological effects of xenobiotics on P. olivacea, providing new sensitive biomarkers of exposure to BBP, BPA, and BP3. It also highlights the suitability of Prodiamesa for ecotoxicological risk assessment, especially in aquatic ecosystems.en
dc.description.versionversión final
dc.identifier.citationLlorente, L., Herrero, Ó., Aquilino, M., & Planelló, R. (2020). Prodiamesa olivacea: de novo biomarker genes in a potential sentinel organism for ecotoxicity studies in natural scenarios. Aquatic Toxicology, 227. https://doi.org/10.1016/j.aquatox.2020.105593
dc.identifier.doihttps://doi.org/10.1016/j.aquatox.2020.105593
dc.identifier.issn0166-445X | eISSN 1879-1514
dc.identifier.urihttps://hdl.handle.net/20.500.14468/25433
dc.journal.titleAquatic Toxicology
dc.journal.volume227
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentFísica Matemática y de Fluídos
dc.rightsinfo:eu-repo/semantics/OpenAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject22 Física
dc.subject.keywordsTranscriptional biomarkeren
dc.subject.keywordsDetoxificationen
dc.subject.keywordsOxidative stressen
dc.subject.keywordsEmergent pollutantsen
dc.subject.keywordsSentinel organismen
dc.subject.keywordsEndocrine disruptoren
dc.titleProdiamesa olivacea: de novo biomarker genes in a potential sentinel organism for ecotoxicity studies in natural scenariosen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication16da09a8-34db-4259-90d7-36928325faff
relation.isAuthorOfPublication1de7b6f9-dd97-4518-8f0e-3c54768bfe6a
relation.isAuthorOfPublication.latestForDiscovery16da09a8-34db-4259-90d7-36928325faff
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