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Prussian Blue/Chitosan Micromotors with Intrinsic Enzyme-like Activity for (bio)-Sensing Assays

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dc.contributor.authorMolinero-Fernández, Águeda
dc.contributor.authorJurado Sánchez, Beatriz
dc.contributor.authorEscarpa, Alberto
dc.contributor.authorMaría Hormigos, Roberto
dc.contributor.authorNovillo López, Miguel Ángel
dc.date.accessioned2024-05-20T11:18:12Z
dc.date.available2024-05-20T11:18:12Z
dc.date.issued2022
dc.description.abstractPrussian Blue (PB)/chitosan enzyme mimetic tubular micromotors are used here for on-the-fly (bio)-sensing assays. The micromotors are easily prepared by direct deposition of chitosan into the pores of a membrane template and in situ PB synthesis during hydrogel deposition. Under judicious pH control, PB micromotors display enzyme mimetic capabilities with three key functions on board: the autonomous oxygen bubble propulsion (with PB acting as a catalase mimic for hydrogen peroxide decomposition), 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation (with PB acting as a peroxidase mimic for analyte detection), and as a magnetic material (to simplify the (bio)-sensing steps). In connection with chitosan capabilities, these unique enzyme mimetic micromotors are further functionalized with acetylthiocholinesterase enzyme (ATChE) to be explored in fast inhibition assays (20 min) for the colorimetric determination of the nerve agent neostigmine, with excellent analytical performance in terms of quantification limit (0.30 μM) and concentration linear range (up to 500 μM), without compromising efficient micromotor propulsion. The new concept illustrated holds considerable potential for a myriad of (bio)-sensing applications, including forensics, where this conceptual approach remains to be explored. Micromotor-based tests to be used in crime scenes are also envisioned due to the reliable neostigmine determination in unpretreated samples.en
dc.description.versionversión publicada
dc.identifier.doihttps://doi.org/10.1021/acs.analchem.1c05173
dc.identifier.issn0003-2700
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11595
dc.journal.issue14
dc.journal.titleAnalytical Chemistry
dc.journal.volume94
dc.language.isoen
dc.publisherACS Publications
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.titlePrussian Blue/Chitosan Micromotors with Intrinsic Enzyme-like Activity for (bio)-Sensing Assayses
dc.typejournal articleen
dc.typeartículoes
dspace.entity.typePublication
relation.isAuthorOfPublication63ba22c0-b75a-41a0-80f8-9a74f39ec038
relation.isAuthorOfPublication8f7360e3-77e4-4a33-8b15-87a7b917cde6
relation.isAuthorOfPublication.latestForDiscovery63ba22c0-b75a-41a0-80f8-9a74f39ec038
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