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Synthesis and characterization of a molecularly imprinted polymer for the determination of spiramycin in sheep milk

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dc.contributor.authorDurand Alegría, J.S.
dc.contributor.authorGarcía Mayor, M Asunción
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorFernández Hernando, Pilar
dc.contributor.authorGarcinuño Martínez, Rosa Mª
dc.date.accessioned2024-05-20T11:18:26Z
dc.date.available2024-05-20T11:18:26Z
dc.date.issued2017-04-15
dc.description.abstractA series of molecularly imprinted polymers (MIPs) comprising reactionary sites which are complementary to macrolide antibiotic spiramycin (SPI) were synthetized by noncovalent bulk polymerization technique. MIPs were synthesized under different polymerization process and their recognition efficiency was evaluated in binding studies in comparison with non-imprinted polymers. The best MIP was morphologically characterized and equilibrium assays were carried out. The MIP was evaluated as a sorbent for extraction and preconcentration of SPI from aqueous and sheep milk samples, and an off-line MISPE method followed by high-performance liquid chromatography with UV diode-array detection was established. Good linearity were obtained for SPI in a range of 24–965 μg kg−1 and the average recoveries at three spiked levels in milk samples were higher than 90% (RSD < 5%). Limit of quantification was 24.1 μg kg−1. Cross-reactivity studies from other macrolides with similar structure were tested. The optimum imprinted polymer showed a good selectivity and affinity for SPI, demonstrating the potential of the proposed MISPE for rapid, sensitive and effective sample pretreatment for selective determination of SPI in sheep milk samples.en
dc.description.versionversión final
dc.identifier.doihttps://doi.org/10.1016/j.foodchem.2016.11.114
dc.identifier.issn0308-8146 - eISSN 1873-7072
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11611
dc.journal.titleFood Chemistry
dc.journal.volume22
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject.keywordsMolecularly imprinted polymer
dc.subject.keywordsSolid phase extraction
dc.subject.keywordsSpiramycin
dc.subject.keywordsSheep milk
dc.titleSynthesis and characterization of a molecularly imprinted polymer for the determination of spiramycin in sheep milkes
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery5e470775-fe11-4279-be7d-7d42172f5413
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