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Permanent oriented antibody immobilization for digoxin determination with a flow-through fluoroimmunosensor

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dc.contributor.authorDurand Alegría, Jesús Senén
dc.contributor.authorPérez Conde, Concepción
dc.contributor.authorFernández Hernando, Pilar
dc.contributor.authorPaniagua González, Gema
dc.date.accessioned2024-05-20T11:18:25Z
dc.date.available2024-05-20T11:18:25Z
dc.date.issued2003-02-28
dc.description.abstractDigoxin is a very important compound in clinical chemistry and is indicated in the treatment of congestive heart failure and artery disease. The measurement of serum digoxin concentration is necessary owing to the narrow therapeutic range of this drug. Further, even with similar dosage regimens, the biological response of patients often results in very different concentrations of digoxin in serum. Concentrations of greater than 2.6 mmol/L are generally interpreted as toxic in adult patients. Most methods for digoxin determination are based on gas chromatography or radiochemical and enzymatic immunoassay techniques. However, some of these methods are tedious and difficult to automate. Nowadays, they are being replaced by more practical immunoassay techniques, involving, for example, fluorescent immunosensors that allow rapid, automated and selective digoxin determinations. This paper reports a new flow–through fluoroimmunosensor for digoxin determination, the function of which is based on antibodies immobilized on an inmunoreactor of controlled pore glass (CPG). The immunosensor has a detection limit of 1.20 μg/L and provides high reproducibility (RSD = 4.5% for a concentration of 0.0025 mg/L, and RSD = 6.7% for 0.01 mg/L). The optimum working concentration range was found to be 1.2×10-3 - 4.0×10-2 mg/L. The lifetime of the immunosensor was about 50 immunoassays, if stored unused its lifetime can be extended to three months. A sample speed of about 10–12 samples per hour can be attained. Possible interference from substances with structures similar to digoxin (morphine, heroine, tebaine, codeine, pentazocine and narcotine) was investigated. No cross-reactivity was seen at the highest digoxin:interferent ratio studied (1:100). The proposed fluoroimmunosensor was successfully used to determine digoxin concentrations in human serum samples.en
dc.description.versionversión final
dc.identifier.doihttps://doi.org/10.1007/s00216-002-1746-4
dc.identifier.issn1618-2642 - eISSN 1618-2650
dc.identifier.urihttps://hdl.handle.net/20.500.14468/11610
dc.journal.titleAnalytical and Bioanalytical Chemistry
dc.journal.volume375
dc.language.isoen
dc.publisherSpringer
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject.keywordsflow-through fluoroimmunosensor
dc.subject.keywordsdigoxin
dc.subject.keywordspermanent immobilization
dc.subject.keywordsserum samples
dc.titlePermanent oriented antibody immobilization for digoxin determination with a flow-through fluoroimmunosensores
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublicationf7c40fc3-8aca-42ef-a900-161d1b1be92e
relation.isAuthorOfPublication6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublication.latestForDiscoveryf7c40fc3-8aca-42ef-a900-161d1b1be92e
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