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Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS

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dc.contributor.authorFernández Pintor, Begoña
dc.contributor.authorGañán Aceituno, Judith
dc.contributor.authorMorante Zarcero, Sonia
dc.contributor.authorSierra, Isabel
dc.contributor.authorPaniagua González, Gema
dc.contributor.authorFernández Hernando, Pilar
dc.contributor.authorGarcinuño Martínez, Rosa Mª
dc.date.accessioned2024-06-11T15:15:06Z
dc.date.available2024-06-11T15:15:06Z
dc.date.issued2024-04-09
dc.description.abstractTwo homopolymers have been prepared employing methacrylic acid and 4-vynylpiridine as functional monomers (p-MAA, p-4-VP) through an easy and quick precipitation polymerization method for application as sorbents in solid-phase extraction (SPE) to determine atropine and scopolamine in honey. The optimized SPE conditions were as follows: 25 mg of p-MAA, 4 mL of sample loading volume (diluted honey 1:10 with water), 4 mL of elution solvent (methanol/water with 1 % formic acid, 80/20, v/v). The extracts were analyzed by HPLC-MS/MS. The cartridges were reusable for forty cycles demonstrating an environmentally friendly approach. The methodology was validated in terms of linearity, accuracy, precision, selectivity, matrix effect and sensibility, highlighting the absence of matrix effect. The miniaturized polymer-based SPE was successfully applied to fifteen honeys, showing concentrations up to 7.23 ng/g in the most contaminated honey. All quantified honey samples (5 in total) were of multifloral type.en
dc.description.versionversión final
dc.identifier.doihttps://doi.org/10.1016/j.polymer.2024.126904
dc.identifier.issn0032-3861
dc.identifier.urihttps://hdl.handle.net/20.500.14468/22314
dc.journal.titlePolymer
dc.journal.volume298
dc.language.isoen
dc.publisherElsevier
dc.relation.centerFacultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0
dc.subject.keywordsTropane alkaloids
dc.subject.keywordsAtropine
dc.subject.keywordsScopolamine
dc.subject.keywordsHoney samples
dc.subject.keywordsSolid-phase extraction
dc.subject.keywordsHPLC-MS/MS
dc.subject.keywordsPolymer
dc.subject.keywordsFood safety
dc.titleDetermination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MSes
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
relation.isAuthorOfPublicationf7c40fc3-8aca-42ef-a900-161d1b1be92e
relation.isAuthorOfPublicationdb6e39ea-d23e-4b0b-94ba-0cd2c62308fb
relation.isAuthorOfPublication.latestForDiscovery6e5a84b2-e6a7-4bb1-9bdc-4e9831d90d11
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