Publicación:
Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

Vista sencilla de ítem
dc.contributor.authorSierra, Tania
dc.contributor.authorHenry, Charles S.
dc.contributor.authorGonzález Crevillén, Agustín
dc.contributor.authorEscarpa, Alberto
dc.date.accessioned2024-10-03T12:02:46Z
dc.date.available2024-10-03T12:02:46Z
dc.date.issued2021-10-25
dc.description.abstractA disposable pump-free electrochemical microfluidic device made by using a multilayer lamination technique is proposed for diagnosis of glycosylation disorders. Thanks to the stacking of polyethylene terephthalate (PET) films and double adhesive layers, it is possible to create a geometry that allows the filling of the tailored channels in a fixed time. In the main channel of this passive microfluidic device, the necessary steps to perform the electrochemical determination of transferrin are performed: labeling of the protein with Os (VI) complex (electrochemical tag), washing with water of the osmium residues and electrochemical detection by adsorptive transfer stripping square wave voltammetry. Electrochemical detection released two electrochemical signals: one from Os (VI) complex due to carbohydrates at 0.9 V, and other from the intrinsic electrochemical signal of glycoprotein due to the amino acids at + 0.8 V. The ratio between them establishes an indicator of the degree of glycosylation (called electrochemical index of glycosylation). The method was successfully applied to the analysis of clinical samples from patients with congenital disorders of glycosylation.en
dc.description.versionversión publicada
dc.identifier.citationTania Sierra, Charles S. Henry, Agustín G. Crevillen, Alberto Escarpa. TÍTULO: “Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation”. Analysis&Sensing, 2022, volumen 2, e202100038 (https://doi.org/10.1002/anse.202100038)
dc.identifier.doihttps://doi.org/10.1002/anse.202100038
dc.identifier.issn2629-2742
dc.identifier.urihttps://hdl.handle.net/20.500.14468/23885
dc.journal.issue1
dc.journal.titleAnalysis&Sensing
dc.journal.volume2
dc.language.isoen
dc.publisherEuropean Chemical Societies Publishing
dc.relation.centerFacultades y escuelas::Facultad de Ciencias
dc.relation.departmentCiencias Analíticas
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/deed.es
dc.subject23 Química::2306 Química orgánica
dc.titleDisposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylationen
dc.typeartículoes
dc.typejournal articleen
dspace.entity.typePublication
relation.isAuthorOfPublication0f39284a-d95a-443a-bb2e-21297ec40b76
relation.isAuthorOfPublication.latestForDiscovery0f39284a-d95a-443a-bb2e-21297ec40b76
Archivos
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
3.62 KB
Formato:
Item-specific license agreed to upon submission
Descripción:
Descargar
Colecciones
Artículos y papers