Persona:
Pellón Suárez de Puga, Ricardo

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0000-0002-4099-7621
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Pellón Suárez de Puga
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Ricardo
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search.filters.applied.f.itemDepartamento: Psicobiología ×

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Mostrando 1 - 2 de 2
  • Miniatura
    Publicación
    Activity-based anorexia alters hypothalamic POMC and orexin populations in male rats
    (Elsevier, 2022-08-11) Pinos, Helena; Sánchez Serrano, Ricardo; Carrillo Urbano, Beatriz; Fernández García, José Manuel; García Úbeda, Rocío; Paz Regidor, Ana María de; López Tolsa Gómez, Gabriela Eugenia; Vidal García, Pedro; Gutiérrez Ferre, Valeria Edith; Pellón Suárez de Puga, Ricardo; Collado Guirao, Paloma
    The objective of this study was to investigate the orexin and POMC populations in the hypothalamic nuclei of male Wistar rats after the activity-based anorexia (ABA) procedure. Four groups were established based on food restriction and activity: activity (A), ABA, diet (D) and control (C). The ABA protocol consisted of free access to a running wheel for a period of 22 h and access to food for 1 h. When the animals in the ABA group reached the ABA criterion, were sacrificed, and their brains were collected and serially sectioned. The free-floating sections were processed for orexin and POMC immunostaining. The number of orexin A-ir cells in the perifornical-dorsomedial-hypothalamus continuum (PFD) and lateral hypothalamus (LH) and the number of POMC-ir cells in the arcuate nucleus (Arc) were estimated. Data on food intake, body weight and wheel turns were also analyzed. The ABA procedure caused a significant decrease in body weight along with a significant increase in activity. Moreover, at the end of the ABA procedure, the number of POMC-ir cells decreased in the Arc in the A group, and significantly more in the ABA group, and the number of orexin A-ir positive cells decreased in the LH in D and ABA groups. The differential decrease in POMC in the ABA group emphasizes the importance of the melanocortin system in the maintenance of ABA, but more research is needed to elucidate the involvement of this peptide in the mechanism that promotes and maintains anorexia nervosa and how increased activity may interact with all these processes.
  • Miniatura
    Publicación
    Influence of early maternal separation on susceptibility to the activity-based anorexia model in male and female Sprague Dawley rats
    (Elsevier, 2022-11) Kojo Morgan, Godstime Stephen; Mata, Yolanda; Carrillo Urbano, Beatriz; Pellón Suárez de Puga, Ricardo; Collado Guirao, Paloma; Gotti, Stefano; Pinos Sánchez, María Elena
    A principal animal paradigm employed in Anorexia Nervosa (AN) study is the activity-based anorexia (ABA) model. The model's efficacy in recapitulating the core features of AN in humans allows for the study of the parameters involved in the disorder. The current study examined the susceptibility to the ABA protocol in the presence of a significant stressor (maternal separation) in male and female Sprague Dawley rats. More importantly, we analysed the sex-differences on activity levels during different periods of the ABA protocol to determine the period(s) influencing the most pathological weight loss. Both components of the ABA protocol contributed to the subjects’ bodyweight loss. Stress in the first two weeks of development conferred a protective effect in males. Time spent and activity levels on the running wheel were higher in females compared to males. Hyperactivity in ABA subjects was observed during the food-anticipatory activity (FAA) and postprandial activity in males and during the FAA and nocturnal activity periods in females. This study aids in understanding the effect of intensity of activity during specific periods on the pathological weight loss in ABA rats. These observations are informative for therapies aimed at ameliorating body mass index in AN patients.