Persona:
Carrillo Urbano, Beatriz

Foto de perfil
Dirección de correo electrónico
ORCID
0000-0002-7393-3675
Fecha de nacimiento
Proyectos de investigación
Unidades organizativas
Puesto de trabajo
Apellidos
Carrillo Urbano
Nombre de pila
Beatriz
Nombre

Filtros

2016 - 20202
Restablecer filtros

Ajustes

Autor: Díaz González, Francisca × Tipo: journal article ×

Resultados de la búsqueda

Mostrando 1 - 2 de 2
  • Miniatura
    Publicación
    Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female Rats
    (Elsevier, 2020) Díaz González, Francisca; Chowen, Julie A.; Grassi, Daniela; Pinos Sánchez, Helena; Carrillo Urbano, Beatriz; Collado Guirao, Paloma
    Estradiol not only participates in the regulation of energy metabolism in adulthood, but also during the first stages of life as it modulates the alterations induced by under- and over-nutrition. The objectives of the present study were to determine: 1) If estradiol is involved in the normal programming of energy metabolism in rats; 2) If there is a specific window of time for this programming and 3) If males and females are differentially vulnerable to the action of this hormone. Estrogen receptors (ER) α, ERβ and GPER were blocked by their specific antagonists MPP, PHTPP and G15, respectively, from postnatal day (P) 1 (the day of birth) to P5 or from P5 to P13. Physiological parameters such as body weight, fat depots and caloric intake were then analysed at P90. Hypothalamic AgRP, POMC, MC4R, ERα, ERβ and GPER mRNA levels and plasma levels of estradiol, were also studied. We found that blocking ER receptors from P5 to P13 significantly decreases long-term body weight in males and hypothalamic POMC mRNA levels in females. The blocking of ERs from P1 to P5 only affected plasma estradiol levels in females. The present results indicate programming actions of estradiol from P5 to P13 on body weight in male and POMC expression in female rats and emphasize the importance of including both sexes in metabolic studies. It is necessary to unravel the mechanisms that underlie the actions of estradiol on food intake, both during development and in adulthood, and to determine how this programming differentially takes place in males and females.
  • Miniatura
    Publicación
    Exposure to increased levels of estradiol during development can have long-term effects on the response to undernutrition in female rats
    (Taylor and Francis Group, 2016-11) Díaz González, Francisca; Chowen, Julie A.; Pino Osuna, María José; Carrillo Urbano, Beatriz; Collado Guirao, Paloma
    Objectives: Undernutrition during development alters the expression of peptides that control energy expenditure and feeding behavior. Estrogens can also modulate these peptides. Here we analyzed whether early postnatal administration of estradiol modulates the effects of undernutrition on neuroendocrine parameters in adult female Wistar rats. Methods: Control rats were fed a control diet. Undernourished pups were submitted to a restricted diet with half of the undernourished rats receiving 0.4 mg/kg s.c. of estradiol benzoate (EB) from postnatal day (P) 6 until P13. Quantitative real-time PCR was performed to determine expression in the hypothalamus of Agouti-related peptide (AgRP), proopiomelanocortin (POMC), neuropeptide Y (NPY) and cocaine- and amphetamine-regulated transcript (CART). Plasma estradiol, testosterone and adiponectin levels were measured by ELISA. Total and acylated ghrelin levels were measured in plasma by RIA. Results: Undernourishment decreased body weight, fat mass, plasma leptin and insulin levels and hypothalamic POMC mRNA levels. An increase in orexigenic signals AgRP and NPY mRNA levels, and in plasma adiponectin levels were found in undernourished animals. Early postnatal treatment with EB to undernourished female rats reversed the effects of undernutrition on adult hypothalamic POMC mRNA levels. In addition, neonatal EB treatment to undernourished females significantly decreased adult plasma testosterone, estradiol and acylated ghrelin levels. Discussion: Our results suggest that increased estradiol during a critical period of development has the capacity to modulate the alterations that undernutrition produces on energy metabolism.