Persona: Martínez Guitarte, José Luis
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0000-0002-7722-864X
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Martínez Guitarte
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José Luis
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Publicación Effects at molecular level of multi-walled carbon nanotubes (MWCNT) in Chironomus riparius (DIPTERA) aquatic larvae(Elsevier, 2019-04) Martínez Paz, Pedro; Negri, Viviana; Esteban Arranz, Adrián; Ballesteros García, Paloma; Martínez Guitarte, José Luis; Morales Camarzana, Consolación MónicaNowadays, due to the physical, chemical, electrical, thermal and mechanical properties of carbon nanotubes (CNT), its have been currently incorporated into biomedical products and they are employed in drug delivery drug administration, biosensor design, microbial treatments, consumer products, and new products containing CNT are expected in the future. CNT are hydrophobic and have a tendency to accumulate in sediments if they are released into aquatic ecosystems. Vertebrate studies have revealed concerns about the toxicity of carbon nanotubes, but there is very limited data on the toxic effects in aquatic invertebrate species. The aim of the present study is to determine the effects of MWCNT in Chironomus riparius at the molecular level, understanding its mode of action and analyzing the suitability of this species to monitor and assess risk of nanomaterials in aquatic ecosystems. To evaluate possible toxic effects caused by carbon nanotube environmental dispersion with regard to aquatic compartment, we study the mRNA levels of several related genes with DNA repairing mechanisms, cell stress response, cell apoptosis and cytoskeleton by Real-Time PCR and proposed a freshwater invertebrate C. riparius, which is a reference organism in aquatic toxicology. The obtained results show a transcriptional alteration of some genes included in this study, indicating that different cell processes are affected and providing one the first evidences in the mechanisms of action of MWCNT in invertebrates. Moreover, this data reinforces the need for further studies to assess the environmental risk of nanomaterial to prevent future damage to aquatic ecosystems.Publicación Effects of single exposure and binary mixtures of ultraviolet filters octocrylene and 2-ethylhexyl 4-(dimethylamino) benzoate on gene expression in the freshwater insect Chironomus riparius(Springer Nature, 2018-10-22) Muñiz González, Ana Belén; Martínez Guitarte, José Luis; Springer NatureUltraviolet filters are used extensively in the production of many personal care and industrial products. These products can inadvertently pollute the environment through recreational activities. They have been associated with endocrine disruption in vertebrates but their effects in invertebrates are poorly understood. Chironomus riparius is a species of the dipteran order, with aquatic larvae that are frequently used in toxicity tests. Previously, we showed that octocrylene (OC) and 2-ethylhexyl 4-(dimethylamino) benzoate (ODPABA) differentially affected the mRNA levels of the ecdysone receptor and Hsp70 genes. For a better understanding of their mode of action, transcriptional activity by real-time PCR was analyzed in fourth instar larvae exposed to OC, OD-PABA, or a binary mixture of both. We studied 16 genes related to the endocrine system, stress, the immune system, and biotransformation mechanisms to elucidate the putative interactions between these compounds. No response was observed for the genes involved in biotransformation, suggesting that enzymes other than cytochromes P450 and glutathione-S-transferases (GSTs) could get involved in transformation of these compounds. Similarly, no response was observed for endocrine-related genes while the stress gene HYOU1 was inhibited by ODPABA, suggesting an effect in response to hypoxia. In addition, no significant interactions were observed following exposure to a binary mixture of these compounds. Overall, the results suggest a weak, acute response in different metabolic pathways and a lack of interaction between the compounds. Finally, new genes are identified in this organism, opening the possibility to analyze new cellular pathways as targets of toxicants.Publicación Colaboraciones en Biología : el láser en Biología, un brillante presente(Universidad Nacional de Educación a Distancia (España). Facultad de Ciencias, 2010-01-01) Planello Carro, María del Rosario; Martínez Guitarte, José LuisPublicación Tesis doctorales: Nuevos doctores de la Facultad de Ciencias(Universidad Nacional de Educación a Distancia (España). Facultad de Ciencias, 2017-01-01) González García, Asunción; Martínez Guitarte, José LuisPublicación Colaboraciones: Las proteínas de estrés. La respuesta de las células a los cambios ambientales(Universidad Nacional de Educación a Distancia (España). Facultad de Ciencias, 2004-01-01) Morcillo Ortega, Gloria; Martínez Guitarte, José LuisPublicación Tesis Doctorales: Nuevos Doctores de la Facultad de Ciencias(Universidad Nacional de Educación a Distancia (España). Facultad de Ciencias, 2019-01-01) Martínez Guitarte, José LuisPublicación Tesis Doctorales: Nuevos Doctores de la Facultad de Ciencias(Universidad Nacional de Educación a Distancia (España). Facultad de Ciencias, 2018-01-01) Martínez Guitarte, José Luis