Persona:
Muñiz González, Ana Belén

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ORCID
0000-0001-8285-7582
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Muñiz González
Nombre de pila
Ana Belén
Nombre

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2022 - 20242
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Ajustes

Autor: Campos, Isabel × Fecha de finalización: 23 ×

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Mostrando 1 - 2 de 2
  • Miniatura
    Publicación
    Effects of wildfire ashes on aquatic invertebrates: First molecular approach on Chironomus riparius larvae
    (Elsevier, 2022-11-03) Muñiz González, Ana Belén; Campos, Isabel; Re, Ana; Martínez Guitarte, José Luis; Nelson Abrantes
    The wildfire magnification in recent years has raised increasing concern about their adverse impacts on the environment. Wildfires are recognized as an important source of diffuse pollution for the nearby aquatic systems being potentially toxic to aquatic life. Albeit previous studies with wildfire runoff/ashes observed effects in aquatic organisms, to date, different severity origins of ashes and their impact at the sub-organismal level on aquatic biota have not been assessed. In this work, the molecular response of Chironomus riparius exposed to wildfire with low (LS) and high (HS) severity ashes from burnt Pine plantations was evaluated by employing an array of 42 genes related to crucial metabolic pathways by Real time-PCR. IV instar larvae were exposed for 72 h to aqueous extract of ashes (12.5 %, 25 %, 50 %, 75 % and 100 %) prepared from LS and HS ashes. Mn, Zn, and Pb were the metals found at highest concentration in both ash extracts, for HS notable Cd, Mn and Cr presence. From the 42 genes studied only 4 were not altered (22 genes modulated their response by LS and 38 genes in the case of HS) showing the opposite response at 100% with downregulated by LS and upregulated by HS. The 12.5 %, 25 %, 100 % HS and 25 % LS were the main modulators, confirmed by the integrative biomarkers response (IBR). Remarkable genotoxicity was generated by ashes even activating the apoptosis response, and endocrine disruption observed could modify the development. Moreover, detoxification and stress response were strongly activated, limiting the organism's future response to external aggressions. The employment of this novelty approach with molecular tools act as early alarm signal preventing greater damages. Overall, wildfire ashes showed to be a significant environmental disruptor to C. riparius even at lower concentration and the short exposure time employed, emphasizing the strong impact of wildfires on aquatic systems.
  • Miniatura
    Publicación
    Towards a comprehensive methodology for ecotoxicological assessment: Prioritizing plant protection products for mixture testing in edge-of-field surface waterbodies
    (Elsevier, 2024-11-06) Abrantes, Nelson; Pereira, Joana Luísa; Muñiz González, Ana Belén; Campos, Isabel; Navarro, Irene; de la Torre, Adrián; Martínez, María Ángeles; Osman, Rima; Jurshid, Chrow Ahmed; Harkes, Paula; Lwanga, Esperanza Huerta; Alcón, Francisco; Contreras, Josefa; Baldi, Isabelle; Oficina, Mathilde; Alaoui, Abdallah; Cristo, Florian; Mandrioli, Daniele; Sgargi, Daria; Paskovic, Igor; Policía Pasković, Marija; Glavan, Matjaž; Hofman, Jakub; Norgaard, Trine; Aparicio, Virginia; Silva, Vera; Elsevier; https://orcid.org/0000-0003-0241-2896; https://orcid.org/0000-0001-7975-8954; https://orcid.org/0000-0002-2942-964X; https://orcid.org/0000-0003-0360-7812; https://orcid.org/0000-0002-3927-3183; https://orcid.org/0000-0002-8280-1199; https://orcid.org/0000-0003-3347-9698; https://orcid.org/0000-0003-3227-6805; https://orcid.org/0000-0003-0738-2673; https://orcid.org/0000-0003-0473-1612; https://orcid.org/0000-0001-6156-6516; https://orcid.org/0000-0003-2153-7213; https://orcid.org/0000-0001-8139-1354; https://orcid.org/0000-0002-9511-6588
    Pesticide applications in agriculture result in complex mixtures of Plant Protection Products (PPPs) in the environment. The ecotoxicological effects of these mixtures can occur at concentrations considered safe for individual chemicals, indicating potential risks underestimated by current regulatory assessments focused on individual active ingredients. To address this challenge, our study introduces a methodology for identifying priority PPPs for formulating mixtures, enabling further ecotoxicological testing in water and sediment compartments of edge-of-field surface water bodies, targeting pelagic and benthic organisms. This methodology was primarily based on the actual quantification of PPPs present in these compartments from selected case study sites (CSSs) in Europe and Argentina (11 and 4 for water and sediments, respectively). A conceptual framework was developed that discriminates and selects concerning PPPs based on their individual risk quotient and frequency of occurrence in each CSS, drawing upon two EU regulatory risk assessment approaches, i.e., the general approach under REACH for any environmental contaminant of concern– the European Chemicals Agency (ECHA) approach; and that specifically focusing on PPPs– the European Food Safety Authority (EFSA) approach. Irrespective of whether the focus is on water or sediments, the study revealed disparities in PPP rankings depending on the approach used to identify PPPs of concern, with the ECHA approach being more conservative than the EFSA approach. Despite this, the EFSA approach follows a more standardized assessment factor definition strategy, potentially allowing avoidance of risk overestimation, as well as resulting in a more balanced representation of different PPP classes for subsequent mixtures testing. Overall, the methodological development reported herein, along with the inconsistencies found when comparing different regulatory approaches to assess the risk of environmental contaminants, highlight the need for further discussion on the most appropriate directions towards the standardization of the regulatory risk assessment of PPP mixtures.