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García Rodríguez, Manuel

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García Rodríguez
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Manuel
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2018 - 201912020 - 20231
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Autor: Sánchez Jiménez, Abel ×

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Mostrando 1 - 2 de 2
  • Miniatura
    Publicación
    Diversity of DNA Sequences from Pathogenic and Potentially Pathogenic Eukaryotic Microorganisms in Protected Granite Mountain Rocks
    (MDPI, 2023) Velasco González, Ismael; Lara, Enrique; Singer, David; Cos Gandoy, Amaya de; Murciano, Antonio; Pérez Uz, Blanca; Williams, Richard; Sánchez Jiménez, Abel; Martín Cereceda, Mercedes; García Rodríguez, Manuel
    Rain-fed mountain granite rock basins are temporary habitats conditioned by a fluctuating environment and the unpredictability of precipitation or flooding rates. These small highland freshwater habitats remain largely unexplored at the microbial level. The aim of this work is to report the presence in these habitats of genetic sequences of microbial eukaryotes that are pathogens and potential pathogens of humans, wildlife, cattle, crops as well as of other microorganisms. We sequenced the hypervariable region v4 of the 18S rDNA gene from environmental DNA of sediments taken from 21 rock basins in a National Park in Spain. More than a fifth (21%) of the eukaryotic Operational Taxonomic Units (OTUs) found are ascribed to pathogenic (within 11 Phyla) and potential pathogenic (within 1 phylum, the Chytridiomycota) microorganisms. Some OTUs retrieved are of agro-economic and public health importance (e.g., Pythium spp., Lagenidium spp., Candida spp. and Vermamoeba vermiformis). In 86% of the basins, the most abundant OTUs were affiliated to Chytridiomycota, a broad fungal group including saprozoic and parasitic taxa. Two OTUs affiliated to chytrids were significantly correlated with high concentrations of heavy metals. The high proportion of chytrid-like microbial sequences found emphasises the role of these freshwater habitats for adding knowledge regarding the ecological trade-offs of the still rather unknown Chytridiomycota. Our results show that rain-fed rock basins may be model habitats for the study and surveillance of microbial community dynamics and genetics of (mainly opportunistic) microbial pathogens.
  • Miniatura
    Publicación
    Complexity of river ciliate communities at a national park highlights the need for microbial conservation
    (John Wiley and Sons, 2018-04) Quintela Alonso, Pablo; Pérez Uz, Blanca; Sánchez Jiménez, Abel; Murciano, Antonio; Centeno, Juan D.; Montero, Esperanza; Muñoz, Benito; Olmedo, Cristina; Refoyo Román, Pablo Alberto; Velasco González, Ismael; Martín Cereceda, Mercedes; García Rodríguez, Manuel
    1. Microorganisms play pivotal roles in aquatic ecosystems. Free‐living protists are the main components of the eukaryotic microbial communities at the base of freshwater ecosystems. Ciliate grazing channels a large proportion of organic matter into multicellular organisms. Surprisingly, ciliates and other microorganisms are neglected in global conservation schemes. 2. Interstitial ciliates were sampled in three sites of varying human pressure on the River Manzanares (La Pedriza National Park, Spain). Abundances of trophic groups and species were adjusted to a generalized linear model (GLM Poisson regression). 3. Ciliate communities were rich in species (74 morphotypes) and although traditional microscopy retrieved a high number of species that appeared only once or in low numbers, rarefaction analyses estimated much larger species richness. These results illustrate that rarefaction assays are a useful first step for exploring the extent of the ciliate cryptic diversity in freshwater ecosystems. 4. Benthic ciliate communities changed significantly, both spatially and at a short temporal scale. The fluctuating nature of the community was manifested by the presence of many ephemeral species at the same river site, revealing a complex and transient community structure. No significant short‐term changes were observed in the physical–chemical properties. Therefore, even slight differences in the abiotic variables may cause rapid shifts of ciliate species. 5. Overall, human pressure had an effect on the interstitial (or benthic) ciliates that resulted in a reduction of species richness and their abundance.