Examinando por Autor "Escarpa, Alberto"

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    Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation
    (European Chemical Societies Publishing, 2021-10-25) Sierra, Tania; Henry, Charles S.; González Crevillén, Agustín; Escarpa, Alberto
    A disposable pump-free electrochemical microfluidic device made by using a multilayer lamination technique is proposed for diagnosis of glycosylation disorders. Thanks to the stacking of polyethylene terephthalate (PET) films and double adhesive layers, it is possible to create a geometry that allows the filling of the tailored channels in a fixed time. In the main channel of this passive microfluidic device, the necessary steps to perform the electrochemical determination of transferrin are performed: labeling of the protein with Os (VI) complex (electrochemical tag), washing with water of the osmium residues and electrochemical detection by adsorptive transfer stripping square wave voltammetry. Electrochemical detection released two electrochemical signals: one from Os (VI) complex due to carbohydrates at 0.9 V, and other from the intrinsic electrochemical signal of glycoprotein due to the amino acids at + 0.8 V. The ratio between them establishes an indicator of the degree of glycosylation (called electrochemical index of glycosylation). The method was successfully applied to the analysis of clinical samples from patients with congenital disorders of glycosylation.
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    Electrochemically Reduced Graphene Oxide-Based Screen-Printed Electrodes for Total Tetracycline Determination by Adsorptive Transfer Stripping Differential Pulse Voltammetry
    (MDPI, 2019-12-21) S. Lorenzetti, Anabela; Sierra, Tania; Domini, Claudia E.; Lista, Adriana G.; González Crevillén, Agustín; Escarpa, Alberto
    Disposable electrochemically reduced graphene oxide-based (ERGO) screen-printed electrodes (SPE) were developed for the determination of total tetracyclines as a sample screening approach. To this end, a selective adsorption-detection approach relied on adsorptive transfer stripping differential pulse voltammetry (AdTDPV) was devised, where the high adsorption capacity and the electrochemical properties of ERGO were simultaneously exploited. The approach was very simple, fast (6 min.), highly selective by combining the adsorptive and the electrochemical features of tetracyclines, and it used just 10 μL of the sample. The electrochemical sensor applicability was demonstrated in the analysis of environmental and food samples. The not-fully explored AdTDPV analytical possibilities on disposable nanostructured transducers become a new tool in food and environmental fields; drawing new horizons for “in-situ” analysis.