Publicación: Electrochemical sensor for the assessment of carbohydrate deficient transferrin: Application to diagnosis of congenital disorders of glycosilation
Cargando...
Fecha
2021-05
Editor/a
Director/a
Tutor/a
Coordinador/a
Prologuista
Revisor/a
Ilustrador/a
Derechos de acceso
info:eu-repo/semantics/openAccess
Título de la revista
ISSN de la revista
Título del volumen
Editor
Elsevier
Resumen
Carbohydrate deficient transferrin (CDT) is used as biomarker of different health problems as, for example, congenital disorders of glycosylation (CDG). We propose a screen-printed-based electrochemical sensor for the determination of carbohydrate deficient transferrin using an Os (VI) tag-based electrochemistry. When transferrin is labeled with Os (VI) complex, it generates two voltammetric signals: one from carbohydrates (electrochemical signal of osmium (VI) complex at -0.9 V/Ag) and one from the amino acids present in glycoprotein (intrinsic electrochemical signal of glycoprotein at +0.8 V/Ag). The relationship between the two analytical signals (carbohydrate signal/protein signal) is an indicator of the degree of glycosylation (electrochemical index of glycosylation), which has shown an excellent correlation (r = 0.990) with the official parameter %CDT obtained by CE-UV. The suitability of this approach was demonstrated by analyzing serum samples from CDG patients.
Descripción
Este es el manuscrito aceptado del artículo. La versión registrada fue publicada por primera vez por Elsevier en Biosensors and Bioelectronics, Volume 179, 2021, 113098; está disponible en línea en el sitio web del editor: https://doi.org/10.1016/j.bios.2021.113098
This is the accepted manuscript of the article. The published version was first published by elsevier in Biosensors and Bioelectronics, Volume 179, 2021, 113098; It is available online at the publisher's website: https://doi.org/10.1016/j.bios.2021.113098
Categorías UNESCO
Palabras clave
Carbohydrate deficient transferrin, Congenital disorders of glycosylation, Screen printed carbon electrode, Electrochemical tag Rare disease
Citación
Tania Sierra, Agustín G. Crevillen, Alberto Escarpa, Electrochemical sensor for the assessment of carbohydrate deficient transferrin: Application to diagnosis of congenital disorders of glycosilation, Biosensors and Bioelectronics, Volume 179, 2021, 113098, https://doi.org/10.1016/j.bios.2021.113098
Centro
Facultades y escuelas::Facultad de Ciencias
Departamento
Ciencias Analíticas