Dortez, SilviaDeGregorio-Rocasolano, NúriaMillán, MònicaGasull, TeresaGonzález Crevillén, AgustínEscarpa, Alberto2025-10-212025-10-212023-07-23Dortez, S., DeGregorio-Rocasolano, N., Millán, M., GasullAgustín, T., G. Crevillen, A., Escarpa, A.(2023).Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients. Analytical Chemistry 2023, 95, 33, 12391–12397. https://doi.org/10.1021/acs.analchem.3c01982eISSN: 1520-6882https://doi.org/10.1021/acs.analchem.3c01982https://hdl.handle.net/20.500.14468/30549The registered version of this article, first published in “Analytical Chemistry 2023, 95, 33, 12391–12397", is available online at the publisher's website: ACS, https://doi.org/10.1021/acs.analchem.3c01982 La versión registrada de este artículo, publicado por primera vez en “Analytical Chemistry 2023, 95, 33, 12391–12390", está disponible en línea en el sitio web del editor: ACS, https://doi.org/10.1021/acs.analchem.3c01982For the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously measured on the same PAD using ferrozine as a chromophore and a smartphone as the color reader. To this end, Tf was first isolated from serum using anti-Tf immunomagnetic beads to ensure that only the Tf-bound iron was measured, improving the selectivity and accuracy of TSAT assessment. To demonstrate the practical utility of the device, it was validated by analyzing a certified reference material, showing excellent accuracy (Er < 4%) and good precision (RSD ≤ 6%). Finally, 18 diagnosed serum samples from ischemic stroke patients were analyzed by this approach, and the results were compared with those obtained by urea-PAGE, showing not only an excellent correlation (r = 0.93, p < 0.05) but that the PAD approach has become statistically identical to the free-interference urea-PAGE. In comparison with the slow, tedious, and non-miniaturized-PAGE, this PAD approach exhibited attractive characteristics such as low cost, disposability, and connectivity, showing great potential for future point-of-care testing, especially in developing countries and/or remote areas, where access to medical or clinical facilities is limited.eninfo:eu-repo/semantics/openAccess2301 Química analítica2306 Química orgánicaartículoColorColorimetryIron SerumTesting and assessment