González Crevillén, AgustínMayorga-Martinez, Carmen C.Vaghasiya, Jayraj V.Martin Pumera2024-10-072024-10-072022-02-05Agustín G. Crevillen, Carmen C. Mayorga-Martinez, Jayraj V. Vaghasiya, Martin Pumera, 3D-Printed SARS-CoV-2 RNA Genosensing Microfluidic System Adv. Mater. Technol. 2022, 7, 2101121; DOI: 10.1002/admt.202101121; https://onlinelibrary.wiley.com/doi/10.1002/admt.2021011212365-709X; e-ISSN: 2365-709Xhttps://doi.org/10.1002/admt.202101121https://hdl.handle.net/20.500.14468/23939Este es el manuscrito aceptado del artículo. La versión registrada fue publicada por primera vez en Advanced Materials Technologies2022, 7, 2101121, está disponible en línea en el sitio web del editor: Wiley: https://doi.org/10.1002/admt.202101121 This is the accepted manuscript of the article. The Version of Record was first published in Advanced Materials Technologies2022, 7, 2101121, it is available online at the publisher's website: Wiley: https://doi.org/10.1002/admt.202101121Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab-on-a-chip device through electrochemical detection using 3D printing technology for SARS-CoV-2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D-printed by a 3D printing pen (3D-PP). The 3D-PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS-CoV-2. The sensing mechanism relies on the electro-oxidation of adenines present in ssDNA when in contact with SARS-CoV-2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS-CoV-2/3D-PP genosensor shows high sensitivity and fast response.eninfo:eu-repo/semantics/openAccess23 Químicaartículo