González Gómez, LorenaMorante Zarcero, SoniaPérez Quintanilla, DamiánPaniagua González, GemaGarcinuño Martínez, Rosa MªFernández Hernando, PilarSierra Alonso, Isabel2024-10-232024-10-232024González-Gómez, L.; Morante-Zarcero, S.; Pérez-Quintanilla, D.; González, G.P.; Garcinuño, R.M.; Hernando, P.F.; Sierra, I. Development and Characterization of SBA-15 Imprinted Polymers for Spiramycin Analysis. Separations 2024, 11, 247. https://doi.org/10.3390/ separations110802472297-8739https://doi.org/10.3390/separations11080247https://hdl.handle.net/20.500.14468/24095The registered version of this article, first published in “Separations 2024, 11, 247", is available online at the publisher's website: MDPI, https://doi.org/10.3390/separations11080247 La versión registrada de este artículo, publicado por primera vez en “Separations 2024, 11, 247", está disponible en línea en el sitio web del editor: MDPI, https://doi.org/10.3390/separations11080247This work focuses on the development of a hybrid material based on SBA-15 silica with a molecularly imprinted polymer (MIP), using spiramycin (SPI) as a template, for use as sorbent in solid-phase extraction (SPE). Characterization techniques such as nitrogen gas adsorption–desorption isotherms, infrared spectroscopy and scanning electron microscopy confirmed the structure and properties of the SBA-15@MIP-SPI material. SPE conditions using SBA-15@MIP-SPI as sorbent were optimized, which allowed us to demonstrate the high selectivity and adsorption capacity of SPI on the synthesized material. The best conditions were 50 mg of sorbent, loading with 1 mL of standard solution or sample of cow milk previously extracted in acetonitrile and eluting with 3 mL of methanol with 1% acetic acid. After the optimization process, the material demonstrated recovery percentages of 81 ± 3% in SPI standard solutions and showed its potential in cow milk samples (71 ± 6%). The novelty of the research consists of the combination of MIPs with SBA-15, which could offer important advantages in terms of specific surface area and porous structure, thus improving performance and reducing the amount of sorbent compared to other traditional methods.eninfo:eu-repo/semantics/openAccess23 QuímicaartículospiramycinSBA-15SBA-15@MIP-SPIhybrid materialmolecularly imprinted polymerssolid-phase extractionSPEselective materialcow milk