Effect of environmental stressors on the mRNA expression of ecdysone cascade genes in Chironomus riparius

Fuente Rubio, Mercedes de laMartín Folgar, RaquelMartínez Paz, PedroCortés Rubio, María Estrella del PerpetuoMartínez Guitarte, José LuisMorales Camarzana, Consolación Mónica2024-10-032024-10-032021-09-13de la Fuente, ., Folgar, R.M., Martínez-Paz, P. et al. Effect of environmental stressors on the mRNA expression of ecdysone cascade genes in Chironomus riparius. Environ Sci Pollut Res 29, 10210–10221 (2022). https://doi.org/10.1007/s11356-021-16339-31614-7499https://doi.org/10.1007/s11356-021-16339-3https://hdl.handle.net/20.500.14468/23892The registered version of this article, first published in Environmental Science and Pollution Research , is available online at the publisher's website: Springer, https://doi.org/10.1007/s11356-021-16339-3La versión registrada de este artículo, publicado por primera vez en Environmental Science and Pollution Research , está disponible en línea en el sitio web del editor: Springer, https://doi.org/10.1007/s11356-021-16339-3Chemical compounds produced by humans are continuously reaching the environment. In this work, we characterised the expression patterns of important endocrine-related genes involved in the ecdysone pathway in the fourth larval instar of the model species Chironomus riparius after exposure to three chemicals: ethinyl oestradiol (EE), nonylphenol (NP) and bis(tributyltin) oxide (TBTO). We used real-time PCR to analyse the gene expression levels of ecdysone receptor (EcR) and ultraspiracle (usp), two genes that encode the dimerising partners of the functional ecdysone receptor; the orphan receptor ERR (oestrogen-related receptor), with an unknown function in invertebrates; and E74, an early response gene induced by ecdysteroids. We estimated the bioaccumulation potential, bioavailability and physicochemical properties of these chemicals, together with a number of other exogenous agents known to interfere with the hormonal system. We also provide a review of previous transcriptional studies showing the effect of all these chemicals on ecdysone cascade genes. This analysis provides useful data for future ecotoxicological studies involving invertebrate species.eninfo:eu-repo/semantics/openAccess24 Ciencias de la VidaEffect of environmental stressors on the mRNA expression of ecdysone cascade genes in Chironomus ripariusartículoendocrine-disrupting chemicals (EDCs)chironomus ripariustoxicitytranscriptional response