Tania, SierraJang, IlhoonNoviana, EkaGonzález Crevillén, AgustínEscarpa, AlbertoS. Henry, Charles2024-10-072024-10-072021-07-05Tania Sierra, Ilhoon Jang, Eka Noviana, Agustín G. Crevillen, Alberto Escarpa, and Charles S. Henry ACS Sensors 2021 6 (8), 2998-3005; https://doi.org/10.1021/acssensors.1c008642379-3694https://doi.org/10.1021/acssensors.1c00864https://hdl.handle.net/20.500.14468/23937Este es el manuscrito aceptado del artículo. La versión registrada fue publicada por American Chemical Society Publications por primera vez en Sensors 2021 6 (8), 2998-3005, está disponible en línea en el sitio web del editor ACS Publications: https://doi.org/10.1021/acssensors.1c00864 This is the accepted manuscript of the article. The published version was first published by American Chemical Society Publications in Sensors 2021 6 (8), 2998-3005, and is available online at the publisher ACS Publications website: https://doi.org/10.1021/acssensors. 1c00864α1-Acid glycoprotein (AGP) is a glycoprotein present in serum, which is associated with the modulation of the immune system in response to stress or injuries, and a biomarker for inflammatory diseases and cancers. Here, we propose a pump-free microfluidic device for the electrochemical determination of AGP. The microfluidic device utilizes capillary-driven flow and a passive mixing system to label the AGP with the Os (VI) complex (an electrochemical tag) inside the main channel, before delivering the products to the electrode surface. Furthermore, thanks to the resulting geometry, all the analytical steps can be carried out inside the device: labeling, washing, and detection by adsorptive transfer stripping square wave voltammetry. The microfluidic device exhibited a linear range from 500 to 2000 mg L–1 (R2 = 0.990) and adequate limit of detection (LOD = 231 mg L–1). Commercial serum samples were analyzed to demonstrate the success of the method, yielding recoveries around 83%. Due to its simplicity, low sample consumption, low cost, short analysis time, disposability, and portability, the proposed method can serve as a point-of-care/need testing device for AGP.eninfo:eu-repo/semantics/openAccess23 Químicaartículocapillary-driven microfluidicpassive mixerα1-acid glycoproteinelectrochemical detectionelectrochemical tag