Marraudino, MarilenaCarrillo Urbano, BeatrizBonaldo, BrigittaLlorente, RicardoCampioli, EliaGarate, IciarPinos Sánchez, María ElenaGarcia Segura, LuisCollado Guirao, PalomaGrassi, Daniela2024-12-302024-12-302021-06-29Marraudino M., Carrillo B., Bonaldo B., Llorente R., Campioli E., Garate I., Pinos H., Garcia-Segura LM., Collado P., Grassi D. (2020). G protein-coupled estrogen receptor immunoreactivity in the rat hypothalamus is widely distributed in neurons, astrocytes and oligodendrocytes, fluctuates during the estrous cycle and is sexually dimorphic. Neuroendocrinology, 111:660-677. FI: 6.804; Q1; https://doi.org/10.1159/0005095830028-3835; e-ISSN: 1423-0194https://doi.org/10.1159/000509583https://hdl.handle.net/20.500.14468/25066Este es el manuscrito aceptado del artículo. La versión registrada fue publicada por primera vez en Neuroendocrinology, 111(7):660-677. FI: 6.804; Q1, está disponible en línea en el sitio web del editor: https://doi.org/10.1159/000509583. This is the accepted manuscript of the article. The proprietary version was first published in Neuroendocrinology, 111(7):660-677. IF: 6,804; Q1, is available online at the publisher's website: https://doi.org/10.1159/000509583.Introduction: The membrane-associated G protein-coupled estrogen receptor 1 (GPER) mediates the regulation by estradiol of arginine-vasopressin immunoreactivity in the supraoptic and paraventricular hypothalamic nuclei of female rats and is involved in the estrogenic control of hypothalamic regulated functions, such as food intake, sexual receptivity, and lordosis behavior. Objective: To assess GPER distribution in the rat hypothalamus. Methods: GPER immunoreactivity was assessed in different anatomical subdivisions of five selected hypothalamic regions of young adult male and cycling female rats: the arcuate nucleus, the lateral hypothalamus, the paraventricular nucleus, the supraoptic nucleus, and the ventromedial hypothalamic nucleus. GPER immunoreactivity was colocalized with NeuN as a marker of mature neurons, GFAP as a marker of astrocytes, and CC1 as a marker of mature oligodendrocytes. Results: GPER immunoreactivity was detected in hypothalamic neurons, astrocytes, and oligodendrocytes. Sex and regional differences and changes during the estrous cycle were detected in the total number of GPER-immunoreactive cells and in the proportion of neurons, astrocytes, and oligodendrocytes that were GPER-immunoreactive. Conclusions: These findings suggest that estrogenic regulation of hypothalamic function through GPER may be different in males and females and may fluctuate during the estrous cycle in females.eninfo:eu-repo/semantics/openAccess61 PsicologíaartículoArcuate nucleuslateral hypothalamusparaventricular nucleussupraoptic nucleusventromedial hypothalamus