Tania SierraGonzález Crevillén, AgustínEscarpa Miguel, Jesús Alberto2024-10-072024-10-072021-05Tania Sierra, Agustín G. Crevillen, Alberto Escarpa, Electrochemical sensor for the assessment of carbohydrate deficient transferrin: Application to diagnosis of congenital disorders of glycosilation, Biosensors and Bioelectronics, Volume 179, 2021, 113098, https://doi.org/10.1016/j.bios.2021.1130980956-5663https://doi.org/10.1016/j.bios.2021.113098https://hdl.handle.net/20.500.14468/23933Este es el manuscrito aceptado del artículo. La versión registrada fue publicada por primera vez por Elsevier en Biosensors and Bioelectronics, Volume 179, 2021, 113098; está disponible en línea en el sitio web del editor: https://doi.org/10.1016/j.bios.2021.113098 This is the accepted manuscript of the article. The published version was first published by elsevier in Biosensors and Bioelectronics, Volume 179, 2021, 113098; It is available online at the publisher's website: https://doi.org/10.1016/j.bios.2021.113098Carbohydrate deficient transferrin (CDT) is used as biomarker of different health problems as, for example, congenital disorders of glycosylation (CDG). We propose a screen-printed-based electrochemical sensor for the determination of carbohydrate deficient transferrin using an Os (VI) tag-based electrochemistry. When transferrin is labeled with Os (VI) complex, it generates two voltammetric signals: one from carbohydrates (electrochemical signal of osmium (VI) complex at -0.9 V/Ag) and one from the amino acids present in glycoprotein (intrinsic electrochemical signal of glycoprotein at +0.8 V/Ag). The relationship between the two analytical signals (carbohydrate signal/protein signal) is an indicator of the degree of glycosylation (electrochemical index of glycosylation), which has shown an excellent correlation (r = 0.990) with the official parameter %CDT obtained by CE-UV. The suitability of this approach was demonstrated by analyzing serum samples from CDG patients.eninfo:eu-repo/semantics/openAccess24 Ciencias de la VidaartículoCarbohydrate deficient transferrinCongenital disorders of glycosylationScreen printed carbon electrodeElectrochemical tag Rare disease