Effects of heroin self‑administration and forced withdrawal on the expression of genes related to the mTOR network in the basolateral complex of the amygdala of male Lewis rats

Ucha, Marcos, Roura‑Martínez, David, Santos‑Toscano, Raquel y et al. . (2022) Effects of heroin self‑administration and forced withdrawal on the expression of genes related to the mTOR network in the basolateral complex of the amygdala of male Lewis rats. Psychopharmacology, Volume 239, pages 2559–2571, (2022)

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Título Effects of heroin self‑administration and forced withdrawal on the expression of genes related to the mTOR network in the basolateral complex of the amygdala of male Lewis rats
Autor(es) Ucha, Marcos
Roura‑Martínez, David
Santos‑Toscano, Raquel
et al.
Materia(s) Psicología
Abstract Rationale The development of substance use disorders involves long-lasting adaptations in specific brain areas that result in an elevated risk of relapse. Some of these adaptations are regulated by the mTOR network, a signalling system that integrates extracellular and intracellular stimuli and modulates several processes related to plasticity. While the role of the mTOR network in cocaine- and alcohol-related disorders is well established, little is known about its participation in opiate use disorders. Objectives To use a heroin self-administration and a withdrawal protocol that induce incubation of heroin-seeking in male rats and study the associated effects on the expression of several genes related to the mTOR system and, in the specific case of Rictor, its respective translated protein and phosphorylation. Results We found that heroin self-administration elicited an increase in the expression of the genes Igf1r, Igf2r, Akt2 and Gsk3a in the basolateral complex of the amygdala, which was not as evident at 30 days of withdrawal. We also found an increase in the expression of Rictor (a protein of the mTOR complex 2) after heroin self-administration compared to the saline group, which was occluded at the 30-day withdrawal period. The activation levels of Rictor, measured by the phosphorylation rate, were also reduced after heroin self-administration, an effect that seemed more apparent in the protracted withdrawal group. Conclusions These results suggest that heroin self-administration under extended access conditions modifies the expression profile of activators and components of the mTOR complexes and show a putative irresponsive mTOR complex 2 after withdrawal from heroin use.
Palabras clave mTOR
Rictor
Heroin
Basolateral complex of the amygdala
Incubation of seeking
Editor(es) Springer
Fecha 2022
Formato application/pdf
Identificador bibliuned:DptoPSIBIO-FPSI-Articulos-Mucha-0003
http://e-spacio.uned.es/fez/view/bibliuned:DptoPSIBIO-FPSI-Articulos-Mucha-0003
DOI - identifier https://doi.org/10.1007/s00213-022-06144-2
ISSN - identifier 0033-3158, eISSN 1432-2072
Nombre de la revista Psychopharmacology
Número de Volumen 239
Página inicial 2559
Página final 2571
Publicado en la Revista Psychopharmacology, Volume 239, pages 2559–2571, (2022)
Idioma eng
Versión de la publicación publishedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Psychopharmacology, is available online at the publisher's website: Springer, https://doi.org/10.1007/s00213-022-06144-2
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Psychopharmacology, está disponible en línea en el sitio web del editor: Springer, https://doi.org/10.1007/s00213-022-06144-2

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Creado: Wed, 31 Jan 2024, 21:33:30 CET