Bisphenol A (BPA) modulates the expression of endocrine and stress response genes in the freshwater snail Physa acuta

Morales, Mónica, Martínez Paz, Pedro, Sánchez Argüello, Paloma, Morcillo, Gloria y Martínez Guitarte José Luis . (2018) Bisphenol A (BPA) modulates the expression of endocrine and stress response genes in the freshwater snail Physa acuta. Ecotoxicology and Environmental Safety, Volume 152, 2018, Pages 132-138

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Título Bisphenol A (BPA) modulates the expression of endocrine and stress response genes in the freshwater snail Physa acuta
Autor(es) Morales, Mónica
Martínez Paz, Pedro
Sánchez Argüello, Paloma
Morcillo, Gloria
Martínez Guitarte José Luis
Materia(s) Ciencias
Abstract Bisphenol A (BPA), a known endocrine disrupting chemical (EDC) that can mimic the action of oestrogens by interacting with hormone receptors, is potentially able to influence reproductive functions in vertebrates and invertebrates. The freshwater pulmonate Physa acuta is a sensitive organism to xenobiotics appropriate for aquatic toxicity testing in environmental studies. This study was conducted to explore the effects of BPA on the Gastropoda endocrine system. The effects following a range of exposure times (5–96 h) to BPA in P. acuta were evaluated at the molecular level by analysing changes in the transcriptional activity of the endocrine-related genes oestrogen receptor (ER), oestrogen-related receptor (ERR), and retinoid X receptor (RXR), as well as in genes involved in the stress response, such as hsp70 and hsp90. Real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis showed that BPA induced a significant increase in the mRNA levels of ER, ERR, and RXR, suggesting that these receptors could be involved in similar pathways or regulation events in the endocrine disruptor activity of this chemical at the molecular level in Gastropoda. Additionally, the hsp70 expression was upregulated after 5 and 72 h of BPA exposures, but hsp90 was only upregulated after 5 h of BPA exposure. Finally, we assessed the glutathione-S-transferase (GST) activity after BPA treatment and found that it was affected after 48 h. In conclusion, these data provide, for the first time, evidences of molecular effects produced by BPA in the endocrine system of Gastropoda, supporting the potential of ER, ERR and RXR as biomarkers to analyse putative EDCs in ecotoxicological studies. Moreover, our results suggest that P. acuta is an appropriate sentinel organism to evaluate the effect of EDCs in the freshwater environment.
Palabras clave Physa acuta
Bisphenol A
Heat shock
Hsp genes
Glutathione-S-Transferase
Editor(es) Elsevier
Fecha 2018-05-15
Identificador bibliuned:DptoFMyF-FCIE-Articulos-Mmorales-0007
http://e-spacio.uned.es/fez/view/bibliuned:DptoFMyF-FCIE-Articulos-Mmorales-0007
DOI - identifier https://doi.org/10.1016/j.ecoenv.2018.01.034
ISSN - identifier 0147-6513
Nombre de la revista Ecotoxicology and Environmental Safety
Número de Volumen 152
Página inicial 132
Página final 138
Publicado en la Revista Ecotoxicology and Environmental Safety, Volume 152, 2018, Pages 132-138
Idioma eng
Versión de la publicación submittedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in "Ecotoxicology and Environmental Safety, Volume 152, 2018, Pages 132-138", is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.ecoenv.2018.01.034
Notas adicionales La versión registrada de este artículo, publicado por primera vez en "Ecotoxicology and Environmental Safety, Volume 152, 2018, Pages 132-138, está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.ecoenv.2018.01.034

 
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Creado: Wed, 24 Jan 2024, 22:49:23 CET