Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)

Martín, Folgar, Raquel, Torres Ruiz, Mónica, Alba, Mercedes de, Cañas Portilla, Ana Isabel, González, M. Carmen y Morales, Mónica . (2022) Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio). Chemosphere Volume 312, Part 1, January 2023, 137077


Título Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
Autor(es) Martín, Folgar, Raquel
Torres Ruiz, Mónica
Alba, Mercedes de
Cañas Portilla, Ana Isabel
González, M. Carmen
Morales, Mónica
Materia(s) Ciencias
Abstract Plastics pose a health hazard to living beings and the environment. Plastic degradation produces nano-sized plastic particles (NPs) that end up in terrestrial and aquatic ecosystems, including oceans, rivers, and lakes. Their presence in air, drinking water, sediments, food, and personal care products leads to a variety of exposure routes for living beings, including humans. The toxicity mechanisms of these nanomaterials (NMs) in living organisms and ecosystems are currently unknown, making it a priority to understand their effects at the molecular and cellular levels. The zebrafish (Zf) (Danio rerio) is a model organism which has a high homology with humans and has been widely used to assess the hazard of different xenobiotics. In this study, the expression changes of different genes in 120 hpf Zf embryos (Zfe) after exposure to polystyrene (PS) NPs (30 nm) at concentrations of 0.1, 0.5 and 3 ppm were investigated. The results showed that the gene encoding heat shock protein (hsp70) was down-regulated in a dose-dependent manner. The genes encoding superoxide dismutase (SOD 1 and SOD 2), apoptotic genes (cas 1 and cas 8) and interleukin 1-β (il1β) were activated at the concentration of 3 ppm PS NP, while the anti-apoptotic gene Bcl2α was inhibited at 0.5 and 3 ppm. In addition, the neurotransmitter-related gene Acetyl-Cholinesterase (ache) was significantly inhibited and the DNA repair genes (gadd45α and rad51) were also down-regulated. In contrast, the mitochondrial metabolism-related gene cox1 did not alter its expression in any of the treatments. Most of the changes in gene expression occurred at the highest concentration of NPs. Overall, the results indicated that NPs generated cellular stress that caused certain alterations in normal gene expression (oxidative stress, apoptotic and inflammatory processes, neurotoxicity and anti-apoptotic proteins), but did not cause any mortality after 120 hpf exposure at the three concentrations assayed. These results highlight the need for further studies investigating the effects, at the molecular level, of these materials in humans and other living organisms.
Editor(es) Elsevier
Fecha 2022-11-02
Formato application/pdf
Identificador bibliuned:DptoFMyF-FCIE-Articulos-Mmorales-0002
http://e-spacio.uned.es/fez/view/bibliuned:DptoFMyF-FCIE-Articulos-Mmorales-0002
DOI - identifier https://doi.org/10.1016/j.chemosphere.2022.137077
fecha fin de embargo 2024-11-03
ISSN - identifier 0045-6535
Nombre de la revista Chemosphere
Número de Volumen 312
Número de Issue 1
Publicado en la Revista Chemosphere Volume 312, Part 1, January 2023, 137077
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/embargoedAccess
Tipo de acceso Acceso embargado
Notas adicionales The registered version of this article, first published in "Chemosphere Volume 312, Part 1, January 2023, 137077", is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.chemosphere.2022.137077
Notas adicionales La versión registrada de este artículo, publicado por primera vez en "Chemosphere Volume 312, Part 1, January 2023, 137077", está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.chemosphere.2022.137077

 
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Creado: Wed, 24 Jan 2024, 20:42:50 CET