Prussian Blue/Chitosan Micromotors with Intrinsic Enzyme-like Activity for (bio)-Sensing Assays

María-Hormigos, Roberto, Molinero-Fernández, Águeda, López, Miguel Ángel, Jurado-Sánchez, Beatriz y Escarpa, Alberto . (2022) Prussian Blue/Chitosan Micromotors with Intrinsic Enzyme-like Activity for (bio)-Sensing Assays. Analytical Chemistry; Volumen: 94 (14); Año: 2022; Páginas: 5575-5582

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Título Prussian Blue/Chitosan Micromotors with Intrinsic Enzyme-like Activity for (bio)-Sensing Assays
Autor(es) María-Hormigos, Roberto
Molinero-Fernández, Águeda
López, Miguel Ángel
Jurado-Sánchez, Beatriz
Escarpa, Alberto
Materia(s) Ciencias
Abstract Prussian Blue (PB)/chitosan enzyme mimetic tubular micromotors are used here for on-the-fly (bio)-sensing assays. The micromotors are easily prepared by direct deposition of chitosan into the pores of a membrane template and in situ PB synthesis during hydrogel deposition. Under judicious pH control, PB micromotors display enzyme mimetic capabilities with three key functions on board: the autonomous oxygen bubble propulsion (with PB acting as a catalase mimic for hydrogen peroxide decomposition), 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation (with PB acting as a peroxidase mimic for analyte detection), and as a magnetic material (to simplify the (bio)-sensing steps). In connection with chitosan capabilities, these unique enzyme mimetic micromotors are further functionalized with acetylthiocholinesterase enzyme (ATChE) to be explored in fast inhibition assays (20 min) for the colorimetric determination of the nerve agent neostigmine, with excellent analytical performance in terms of quantification limit (0.30 μM) and concentration linear range (up to 500 μM), without compromising efficient micromotor propulsion. The new concept illustrated holds considerable potential for a myriad of (bio)-sensing applications, including forensics, where this conceptual approach remains to be explored. Micromotor-based tests to be used in crime scenes are also envisioned due to the reliable neostigmine determination in unpretreated samples.
Editor(es) ACS Publications
Fecha 2022
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Rmaria-0003
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Rmaria-0003
DOI - identifier https://doi.org/10.1021/acs.analchem.1c05173
ISSN - identifier 0003-2700
Nombre de la revista Analytical Chemistry
Número de Volumen 94
Número de Issue 14
Página inicial 5575
Página final 5582
Publicado en la Revista Analytical Chemistry; Volumen: 94 (14); Año: 2022; Páginas: 5575-5582
Idioma eng
Versión de la publicación publishedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Analytical Chemistry, is available online at the publisher's website: ACS Publications, https://doi.org/10.1021/acs.analchem.1c05173
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Analytical Chemistry, está disponible en línea en el sitio web del editor: ACS Publications, https://doi.org/10.1021/acs.analchem.1c05173

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Creado: Wed, 24 Jan 2024, 19:29:28 CET