Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change

Monago Maraña, Olga, Muñoz de la Peña, Arsenio, Galeano Díaz , Teresa y Muñoz de la Peña, Arsenio . (2016) Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change. Journal of Agricultural and Food Chemistry

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Título Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
Autor(es) Monago Maraña, Olga
Muñoz de la Peña, Arsenio
Galeano Díaz , Teresa
Muñoz de la Peña, Arsenio
Materia(s) Ciencias
Abstract The determination of flavonoid compounds in paprika samples has been performed by liquid chromatography in series diode array and fluorescence detection (LC–DAD–FLD), by means of a pH change to basic medium just before FLD detection. The validation of the method was performed through the establishment of the external standard calibration curves and the analytical figures of merit. Limits of detection ranging from 0.006 to 0.02 mg L−1 and 0.007 to 0.09 mg L−1 were achieved using DAD and FLD detection, respectively. The experimental conditions to carry out the hydrolysis procedure to obtain flavonoid aglycones from flavonoid glycosides have been optimized applying an experimental design and the response surface methodology. The final conditions selected were 2.5 M HCl during 45 min at 85 °C. The repeatability of this procedure was assayed and relative standard deviation (RSD) values for concentration of quercetin and luteolin compounds were lower than 2%. The quantification of quercetin, luteolin and kaempferol compounds was carried out in less than 6 min in paprika samples by means of the external standard calibration. The analytes were extracted with methanol and the extracts were previously subjected to a cleanup procedure to extend the use of the chromatographic column.
Palabras clave Liquid chromatography
Fluorescence detection
Paprika
Acid hydrolysis
Protected Designation of Origin
Multidimensional-partial least-squares with residual bilinearization
Editor(es) Elsevier
Fecha 2016-05-15
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Omonago-0011
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Omonago-0011
DOI - identifier https://doi.org/10.1016/j.talanta.2016.01.041
ISSN - identifier 0039-9140; eISSN 1873-3573
Nombre de la revista Talanta
Número de Volumen 152
Número de Issue 43
Página inicial 15
Página final 22
Publicado en la Revista Journal of Agricultural and Food Chemistry
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Talanta, está disponible en línea en el sitio web del editor: Elsevier, https://doi.org/10.1016/j.talanta.2016.01.041
Notas adicionales The registered version of this article, first published in Talanta, is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.talanta.2016.01.041

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Creado: Tue, 20 Feb 2024, 19:53:53 CET