Diversity of DNA Sequences from Pathogenic and Potentially Pathogenic Eukaryotic Microorganisms in Protected Granite Mountain Rocks

Velasco-González, Ismael, Lara, Enrique, Singer, David, Cos-Gandoy, Amaya de, García-Rodríguez, Manuel, Murciano, Antonio, Pérez-Uz, Blanca, Williams, Richard, Sanchez-Jimenez, Abel y Martín-Cereceda, Mercedes . (2023) Diversity of DNA Sequences from Pathogenic and Potentially Pathogenic Eukaryotic Microorganisms in Protected Granite Mountain Rocks. Diversity 2023, 15(5), 594

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Título Diversity of DNA Sequences from Pathogenic and Potentially Pathogenic Eukaryotic Microorganisms in Protected Granite Mountain Rocks
Autor(es) Velasco-González, Ismael
Lara, Enrique
Singer, David
Cos-Gandoy, Amaya de
García-Rodríguez, Manuel
Murciano, Antonio
Pérez-Uz, Blanca
Williams, Richard
Sanchez-Jimenez, Abel
Martín-Cereceda, Mercedes
Materia(s) Ciencias
Abstract Rain-fed mountain granite rock basins are temporary habitats conditioned by a fluctuating environment and the unpredictability of precipitation or flooding rates. These small highland freshwater habitats remain largely unexplored at the microbial level. The aim of this work is to report the presence in these habitats of genetic sequences of microbial eukaryotes that are pathogens and potential pathogens of humans, wildlife, cattle, crops as well as of other microorganisms. We sequenced the hypervariable region v4 of the 18S rDNA gene from environmental DNA of sediments taken from 21 rock basins in a National Park in Spain. More than a fifth (21%) of the eukaryotic Operational Taxonomic Units (OTUs) found are ascribed to pathogenic (within 11 Phyla) and potential pathogenic (within 1 phylum, the Chytridiomycota) microorganisms. Some OTUs retrieved are of agro-economic and public health importance (e.g., Pythium spp., Lagenidium spp., Candida spp. and Vermamoeba vermiformis). In 86% of the basins, the most abundant OTUs were affiliated to Chytridiomycota, a broad fungal group including saprozoic and parasitic taxa. Two OTUs affiliated to chytrids were significantly correlated with high concentrations of heavy metals. The high proportion of chytrid-like microbial sequences found emphasises the role of these freshwater habitats for adding knowledge regarding the ecological trade-offs of the still rather unknown Chytridiomycota. Our results show that rain-fed rock basins may be model habitats for the study and surveillance of microbial community dynamics and genetics of (mainly opportunistic) microbial pathogens.
Palabras clave chytrid fungi
ephemeral habitats
microbial eukaryotic pathogens
mountain rock basins
toxic metals
Editor(es) MDPI
Fecha 2023
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Mgarcia-0006
DOI - identifier https://doi.org/10.3390/d15050594
ISSN - identifier 1424-2818
Nombre de la revista Diversity
Número de Volumen 15
Número de Issue 5
Publicado en la Revista Diversity 2023, 15(5), 594
Idioma eng
Versión de la publicación publishedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by/4.0
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Diversity, is available online at the publisher's website: MDPI, https://doi.org/10.3390/d15050594
Notas adicionales La versión registrada de este artículo, publicado por primera vez en Diversity, está disponible en línea en el sitio web del editor: MDPI, https://doi.org/10.3390/d15050594

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Creado: Thu, 25 Jan 2024, 20:48:40 CET