On-line flow injection molecularly imprinted solid phase extraction for the preconcentration and determination of 1-hydroxypyrene in urine samples

Serrano, Montserrat, Bartolomé, Mónica, Bravo, Juan Carlos, Paniagua, Gema, Gañan, Judith, Gallego-Picóa, Alejandrina y Garcinuño, Rosa María . (2017) On-line flow injection molecularly imprinted solid phase extraction for the preconcentration and determination of 1-hydroxypyrene in urine samples. Talanta Vol. 166, Pages 375-382

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Título On-line flow injection molecularly imprinted solid phase extraction for the preconcentration and determination of 1-hydroxypyrene in urine samples
Autor(es) Serrano, Montserrat
Bartolomé, Mónica
Bravo, Juan Carlos
Paniagua, Gema
Gañan, Judith
Gallego-Picóa, Alejandrina
Garcinuño, Rosa María
Materia(s) Ciencias
Abstract New analytical strategies tend to automation of sample pre-treatment and flow analysis techniques provided a number of enhanced analytical methods allowing high throughput. Flow techniques are usually faster, more robust and more flexible than their batch equivalents. In addition, flow methods use less sample and reagent amounts and reduce analytical costs and waste. A flow injection solid-phase extraction pre-concentration system using a molecularly imprinted polymer (MIP) packed micro-column was developed for the determination of 1-hydroxypyrene in human urine with fluorescence detection. The pre-concentration of 1-hydroxypyrene on the MIP was carried out based on the specific retention of analyte by on-line introducing the sample into the micro-column system. Methanol and dichloromethane mixture was used to elute the retained analyte for fluorometric analysis. Important influencing factors were studied in detail, in batch and in flow (MISPE procedure optimisation, sample and eluent volumes, flow rate, dimensions of MIP micro-column and amounts of packing material, etc). To the best of our knowledge, this is the first on-line flow injection molecularly imprinted solid phase extraction for the pre-concentration and determination of hydroxylate PAH metabolite in urine samples. The optimised method was successfully applied to the determination of 1-Hydroxypyrene in spiked urine samples, with recoveries in the range of 74–85% and RSD<4.6%. Under optimum experimental conditions, the linearity concentration range used was 10–400 μg L−1, R2>0.996. We obtained limit of detection and quantification of 3.1 μg L−1 and 10.5 μg L−1, respectively
Palabras clave 1-Hydroxypyrene
On-line solid-phase extraction
Molecularly imprinted polymer
Human urine
Flow injection analysis
Editor(es) Elsevier
Fecha 2017-05-01
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Jcbravo-0003
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Jcbravo-0003
DOI - identifier https://doi.org/10.1016/j.talanta.2016.01.048
ISSN - identifier 0039-9140
Nombre de la revista Talanta
Número de Volumen 166
Página inicial 375
Página final 382
Publicado en la Revista Talanta Vol. 166, Pages 375-382
Idioma eng
Versión de la publicación publishedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The published version of this article, first published in Talanta, is available online at the publisher's website: Elservier, https://doi.org/10.1016/j.talanta.2016.01.048
Notas adicionales La versión publicada de este artículo, publicado por primera vez en Talanta, está disponible en línea en el sitio web del editor: Elservier, https://doi.org/10.1016/j.talanta.2016.01.048

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Creado: Wed, 10 Jan 2024, 22:54:55 CET