Assessment of molecularly imprinted polymers as selective solid-phase extraction sorbents for the detection of cloxacillin in drinking and river water

Garcinuño, Rosa María, Collado, Eduardo José, Paniagua, Gema, Bravo, Juan Carlos y Fernández Hernando, Pilar . (2023) Assessment of molecularly imprinted polymers as selective solid-phase extraction sorbents for the detection of cloxacillin in drinking and river water. Polymers 2023 vol.15 (21), 4314

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Título Assessment of molecularly imprinted polymers as selective solid-phase extraction sorbents for the detection of cloxacillin in drinking and river water
Autor(es) Garcinuño, Rosa María
Collado, Eduardo José
Paniagua, Gema
Bravo, Juan Carlos
Fernández Hernando, Pilar
Materia(s) Ciencias
Química
Abstract This paper describes a new methodology for carrying out quantitative extraction of cloxacillin from drinking and river water samples using a molecularly imprinted polymer (MIP) as a selective sorbent for solid-phase extraction (MISPE). Several polymers were synthesized via thermal polymerization using cloxacillin as a template, methacrylic acid (MAA) as a functional monomer, ethyleneglycoldimethacrylate (EGDMA) as a cross-linker and different solvents as porogens. Binding characteristics of the adequate molecularly imprinted and non-imprinted (NIP) polymers were evaluated via batch adsorption assays following the Langmuir and Freundlich isotherms and Scatchard assays. The parameters related to the extraction approach were studied to select the most appropriate polymer for cloxacillin determination. Using the optimized MIP as the SPE sorbent, a simple sample treatment methodology was combined with high-performance liquid chromatography (HPLC) to analyze cloxacillin residues in drinking and river water. Under the optimum experimental conditions, the MISPE methodology was validated using spiked samples. The linearity for cloxacillin was assessed within the limits of 0.05–1.5 g L􀀀1 and the recovery percentage was higher than 98% (RSD < 4%). The limits of detection and limits of quantification were 0.29 and 0.37 g L􀀀1 and 0.8 and 0.98 g L􀀀1 for drinking and river water, respectively. The selectivity of MIP against other ß-lactam antibiotics with similar structures (oxacillin, cefazoline, amoxicillin and penicillin V) was studied, obtaining a good recovery higher than 85% for all except cefazoline. The proposed MISPE-HPLC methodology was successfully applied for the detection of cloxacillin in drinking water from Canal de Isabel II (Madrid) and river water from the Manzanares River (Madrid).
Palabras clave cloxacillin
molecularly imprinted polymer
solid-phase extraction
adsorption isotherms
water
HPLC-DAD
Editor(es) MDPI
Fecha 2023-11-03
Identificador bibliuned:DptoCA-FCIE-Articulos-Jcbravo-0001
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Jcbravo-0001
DOI - identifier https://doi.org/10.3390/polym15214314
ISSN - identifier 2073-4360
Nombre de la revista Polymers
Número de Volumen 15
Número de Issue 21
Publicado en la Revista Polymers 2023 vol.15 (21), 4314
Idioma eng
Versión de la publicación publishedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published by MDPI in "Polymers" on 3 november 2023, available online: https://doi.org/10.3390/polym15214314
Notas adicionales La versión registrada de este artículo, publicado por primera vez por MDPI en "Polymers" en 3 noviembre 2023, disponible online: https://doi.org/10.3390/polym15214314

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Creado: Thu, 23 Nov 2023, 20:34:09 CET