Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS

Fernández-Pintor, Begoña, Paniagua, Gema, Gañán, Judith, Morante-Zarcero, Sonia, Garcinuño, Rosa María, Fernández, Pilar y Sierra, Isabel . (2024) Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS. Polymer

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Título Determination of atropine and scopolamine in honey using a miniaturized polymer-based solid-phase extraction protocol prior to the analysis by HPLC-MS/MS
Autor(es) Fernández-Pintor, Begoña
Paniagua, Gema
Gañán, Judith
Morante-Zarcero, Sonia
Garcinuño, Rosa María
Fernández, Pilar
Sierra, Isabel
Materia(s) Ciencias
Abstract Two homopolymers have been prepared employing methacrylic acid and 4-vynylpiridine as functional monomers (p-MAA, p-4-VP) through an easy and quick precipitation polymerization method for application as sorbents in solid-phase extraction (SPE) to determine atropine and scopolamine in honey. The optimized SPE conditions were as follows: 25 mg of p-MAA, 4 mL of sample loading volume (diluted honey 1:10 with water), 4 mL of elution solvent (methanol/water with 1 % formic acid, 80/20, v/v). The extracts were analyzed by HPLC-MS/MS. The cartridges were reusable for forty cycles demonstrating an environmentally friendly approach. The methodology was validated in terms of linearity, accuracy, precision, selectivity, matrix effect and sensibility, highlighting the absence of matrix effect. The miniaturized polymer-based SPE was successfully applied to fifteen honeys, showing concentrations up to 7.23 ng/g in the most contaminated honey. All quantified honey samples (5 in total) were of multifloral type.
Palabras clave Tropane alkaloids
Atropine
Scopolamine
Honey samples
Solid-phase extraction
HPLC-MS/MS
Polymer
Food safety
Editor(es) Elsevier
Fecha 2024-04-09
Formato application/pdf
Identificador bibliuned:DptoCA-FCIE-Articulos-Gpaniagua-0019
http://e-spacio.uned.es/fez/view/bibliuned:DptoCA-FCIE-Articulos-Omonago-0019
DOI - identifier https://doi.org/10.1016/j.polymer.2024.126904
ISSN - identifier 0032-3861
Nombre de la revista Polymer
Número de Volumen 298
Publicado en la Revista Polymer
Idioma eng
Versión de la publicación acceptedVersion
Tipo de recurso Article
Derechos de acceso y licencia http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
Tipo de acceso Acceso abierto
Notas adicionales The registered version of this article, first published in Polymer, is available online at the publisher's website: Elsevier, https://doi.org/10.1016/j.polymer.2024.126904

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Creado: Tue, 19 Mar 2024, 19:43:36 CET